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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,537 results for: BASE

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Showing: 1341 - 1360 of 13537 results

  12. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172835-plasmid-map-sequence-id-341082

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  14. MyoUTipGAPi

    Plasmid
    #127549
    Purpose
    Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment
    Depositor
    Luis Vidali
    Article
    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00
    Insert
    Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
    Use
    RNAi; Positive control
    Promoter
    Ubiquitin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    127549-plasmid-map-sequence-id-249090

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  17. pFP59

    Plasmid
    #247215
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from an E. coli sigma70 promoter. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    Pj23100 (ttgacggctagctcagtcctaggtacagtgctagc)
    Available Since
    May 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    247215-plasmid-map-sequence-id-511632

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  18. pFP60

    Plasmid
    #247216
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from an E. coli sigma70 promoter. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    Pj23100 (ttgacggctagctcagtcctaggtacagtgctagc)
    Available Since
    May 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    247216-plasmid-map-sequence-id-511631

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  19. pFP34

    Plasmid
    #247209
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from a T7 promoter. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247209-plasmid-map-sequence-id-507802

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  20. pFP34∆RBS

    Plasmid
    #247213
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from a T7 promoter but lacks a ribosomal binding site. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247213-plasmid-map-sequence-id-507799

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Showing: 1341 - 1360 of 13537 results