We narrowed to 12,549 results for: NUC

1. GFP-p53 (NES-)

   Plasmid

   #12092

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (NES-) (TP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L348A L350A Localized to nucleus.

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-ST1n-VP64

   Plasmid

   #48675

   Purpose

   Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null (D9A D598A H599A N622A)

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-H2A-TORCAR

   Plasmid

   #175016

   Purpose

   TORCAR targeted to the nucleus via histone H2A.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   H2A-TORCAR
   

   Use

   Tags

   Histone H2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SARS-CoV-2_N_deltalinker

   Plasmid

   #157870

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaliner

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SARS-CoV-2_N_deltaCTD

   Plasmid

   #157871

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaCTD

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK506

   Plasmid

   #155382

   Purpose

   dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. M-SPn-VP64

   Plasmid

   #48674

   Purpose

   Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

8. B23(9-122)

   Plasmid

   #23142

   Depositor

   SeWon Suh

   Article

   Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

   Insert

   B23 core (NPM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-8

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pYZ714

   Plasmid

   #132955

   Purpose

   crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   dFnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   adh1

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SARS-CoV-2_N_deltaNTD

    Plasmid

    #157869

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaNTD

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SARS-CoV-2_N_deltaNIDR

    Plasmid

    #157868

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaNIDR

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcGFP-Jpx9C104T

    Plasmid

    #120381

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321T

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-RelA(K221R)

    Plasmid

    #23248

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K221R

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCMV HDM2(NES-)

    Plasmid

    #12087

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nuclear-export sequence mutant (NES-): L205A, I20…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

16. T7-RelA(K218R)

    Plasmid

    #23247

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA(K218R) (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K218R

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SARS-CoV-2_N_deltaCIDR

    Plasmid

    #157872

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaCIDR

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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