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pLL3.7-CAG-hMGT-Ef1a-puro

Plasmid

#172395

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7

Plasmid

#221555

Purpose

Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-WPRE

Plasmid

#202611

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7-UBCp-hMGT-Ef1a-puro

Plasmid

#172396

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA2-IRES-Puro

Plasmid

#61063

Purpose

lentiviral vector for GATA2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA2 (GATA2 Human)

Use

Lentiviral

Tags

HA

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

Plasmid

#35497

Purpose

AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Has Service

AAV9

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E122T and E162T

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-PPO-Venus

Plasmid

#139505

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV8 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Promoter

Ef1a

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Neo-EF1A>Tet3G

Plasmid

#184379

Purpose

The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Tet3G

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-W3SL

Plasmid

#218744

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

Plasmid

#39454

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CD (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-FAS-TdTomato-WPRE-pA

Plasmid

#37092

Purpose

Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-LMO2-IRES-Puro

Plasmid

#61064

Purpose

lentiviral vector for LMO2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

LMO2 (LMO2 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.BB.28.3z

Plasmid

#200681

Purpose

Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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