We narrowed to 2,567 results for: gem

1. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. hMLKL-Venus-Plasma

   Plasmid

   #106081

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   hMLKL-Venus-Plasma (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   plasma membrane-targetting domain of H-Ras (aa 1-…

   Expression

   Mammalian

   Promoter

   dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-HA-CCPG1

   Plasmid

   #139867

   Purpose

   Lentiviral expression of HA-tagged human CCPG1

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CCPG1 (CCPG1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. hMLKL NB(1-182)-2xFv-flag

   Plasmid

   #106075

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-182)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-182

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-HLA-cmyc-natT1R2

   Plasmid

   #113946

   Purpose

   mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CamKII-GFP-P2A-bReaches-minWPRE

   Plasmid

   #118276

   Purpose

   Expresses EGFP and bReaChES under a minimal CamKII Promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   EGFP
   bReaCHES
   

   Use

   AAV

   Tags

   P2A

   Promoter

   CamK II

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mDlx-BiPOLES-mCerulean

   Plasmid

   #154946

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   minimal Dlx

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. hMLKL NB(1-140)-2xFv-flag

   Plasmid

   #106074

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. hMLKL NB(1-140)-2xFv-Venus

   Plasmid

   #106076

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MultiMate 5 colors

    Plasmid

    #206266

    Purpose

    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    

    Use

    Recombinant baculovirus production, cre acceptor…

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-HLA-Flag-natT1R2

    Plasmid

    #113947

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 22-839

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV hSyn1 YFP-CaRhAC

    Plasmid

    #101721

    Purpose

    Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Insert

    YFP CaRhAC
    

    Use

    AAV

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-HLA-cmyc-optiT1R3 a21-852

    Plasmid

    #113948

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-Aion-citrine

    Plasmid

    #192569

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_FLAG-NFL(R438TAG)

    Plasmid

    #182662

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    R438TAG

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. J-sRNA-331Bb

    Plasmid

    #78285

    Purpose

    An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    j-sRNA-331
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MIGR1_FLAG_TAL1-long_GFP

    Plasmid

    #210638

    Purpose

    Overexpression of TAL1-long

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1-short (TAL1 Human)
    

    Use

    Retroviral

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSyn-Aion-ts-citrine-ER

    Plasmid

    #192575

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-Citrine-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. QSOX1-luciferase

    Plasmid

    #113352

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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