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We narrowed to 2,070 results for: gem

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Showing: 1341 - 1360 of 2070 results

  4. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

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  5. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  8. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid
    #160568
    Purpose
    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Publication
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    M1_1-pTRNA-scf 2.1
    Use
    CRISPR
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160568-plasmid-map-sequence-id-319683

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  9. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid
    #160567
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160567-plasmid-map-sequence-id-319681

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  10. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid
    #221447
    Purpose
    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus
    Depositor
    Daniel Durocher
    Publication
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    SPR degron: hGEM (1-110) fragment that is fused t…
    Promoter
    CMV
    Available since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221447-plasmid-map-sequence-id-437531

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  11. STK032_pSTK-0A-PxylA

    Plasmid
    #235411
    Purpose
    Constitutive promoter. (NOTE: Xylose-inducible promoter (1% xylose) when XylR present. See STK161.
    Depositor
    Tom Ellis
    Insert
    PxylA
    Expression
    Bacterial
    Mutation
    No
    Available since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    235411-plasmid-map-sequence-id-504268

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  12. STK070_pSTK-0D-B0015

    Plasmid
    #235369
    Purpose
    Very strong terminator in B. subtilis. Strong transcriptional double terminator in E. coli
    Depositor
    Tom Ellis
    Insert
    Bba_B0015
    Expression
    Bacterial
    Mutation
    No
    Available since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    235369-plasmid-map-sequence-id-504266

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  13. STK180_pSTK-0B-50KRBSGFP

    Plasmid
    #235437
    Purpose
    Synthetic RBS with upstream RiboJ for high translation of GFP
    Depositor
    Tom Ellis
    Insert
    50KRBSGFP
    Expression
    Bacterial
    Mutation
    No
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    235437-plasmid-map-sequence-id-507805

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  14. STK077_pSTK-0D-L3S2P21

    Plasmid
    #235412
    Purpose
    Very strong terminator in B. subtilis. Strongest synthetic transcriptional terminator in E. coli. Recombination resistant
    Depositor
    Tom Ellis
    Insert
    L3S2P21
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    235412-plasmid-map-sequence-id-504270

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  15. STK069_pSTK-0D-B0012

    Plasmid
    #235410
    Purpose
    Strong terminator in B. subtilis. Weak transcriptional terminator in E. coli
    Depositor
    Tom Ellis
    Insert
    Bba_B0012
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235410-plasmid-map-sequence-id-502666

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  16. STK078_pSTK-0D-L3S3P21b

    Plasmid
    #235413
    Purpose
    Very strong terminator in B. subtilis. Strong synthetic transcriptional terminator in E. coli. Recombinant resistant. Modified to remove BsaI site (Position 49G to 49T).
    Depositor
    Tom Ellis
    Insert
    L3S3P21
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235413-plasmid-map-sequence-id-502667

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  17. STK204_pSTK0-B-RBS0

    Plasmid
    #235383
    Purpose
    Very strong RBS in B. subtilis
    Depositor
    Tom Ellis
    Insert
    RBS0
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235383-plasmid-map-sequence-id-502641

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  18. STK205_pSTK0-B-RBS2

    Plasmid
    #235384
    Purpose
    Strong RBS
    Depositor
    Tom Ellis
    Insert
    RBS2
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235384-plasmid-map-sequence-id-502642

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  19. STK046_pSTK-0B-TM_RBS2

    Plasmid
    #235380
    Purpose
    Weak RBS in B. subtilis. Do not confuse with RBS2 (strong)
    Depositor
    Tom Ellis
    Insert
    TM_RBS2
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235380-plasmid-map-sequence-id-502638

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  20. STK048_pSTK-0B-TM_RBS4

    Plasmid
    #235381
    Purpose
    Weak RBS in B. subtilis
    Depositor
    Tom Ellis
    Insert
    TM_RBS4
    Expression
    Bacterial
    Mutation
    No
    Available since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235381-plasmid-map-sequence-id-502639

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Showing: 1341 - 1360 of 2070 results