We narrowed to 5,839 results for: pCas

1. pX330_rActb sgRNA / hSpCas9

   Plasmid

   #172833

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-gamma3 (in pCAGGS)

   Plasmid

   #118042

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_Tuba1b sgRNA / hSpCas9

   Plasmid

   #172835

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-BsCas12b-2AeGFP

   Plasmid

   #121948

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   BsCas12b
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-hCD4-GCaMP2

   Plasmid

   #18932

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   hCD4-GCaMP2 (CD4 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-HDAC4 C-terminus

   Plasmid

   #71400

   Purpose

   CAG promoter, mammalian expression

   Depositor

   Bo Chen

   Article

   Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

   Insert

   HDAC4 C-terminus (HDAC4 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG/hArf4(WT)-EGFP

   Plasmid

   #79407

   Purpose

   Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pGL3-B-838;+80pCalb2

   Plasmid

   #66750

   Purpose

   luciferase reporter for Calb2 promoter (-838 to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-838bp +80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-S-501V2-FLAG

    Plasmid

    #177096

    Purpose

    501V2 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein 501V2 (S SARS-CoV-2)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

    Plasmid

    #107728

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-ChBtW-eSpCas9

    Plasmid

    #133370

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-S-B.1.617.1-FLAG

    Plasmid

    #177098

    Purpose

    B.1.617.1 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.617.1 (S SARS-CoV-2)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFREE2-eSpCas9-sgRNA

    Plasmid

    #179580

    Purpose

    Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    eSpCas9
    

    Expression

    Bacterial

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HDAC4 1-747

    Plasmid

    #71399

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 1-747 (HDAC4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pBT225_(pCA-tdT3Myc)

    Plasmid

    #36873

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-SRGAP3FBAR-EGFP

    Plasmid

    #168506

    Purpose

    Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3-FBAR (SRGAP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-492

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-AmCas12b-2AeGFP

    Plasmid

    #121947

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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