We narrowed to 2,582 results for: pCas

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CP1041-ABE8e

Plasmid

#138493

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (CP1041)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_429

Plasmid

#179098

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (Cas9-NG)

Use

CRISPR and Lentiviral

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAEL010097-Cas9

Plasmid

#100707

Purpose

The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL010097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL010097 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-sgR26

Plasmid

#127376

Purpose

Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

CRISPR and Mouse Targeting

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mTfeb

Plasmid

#79006

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

sgTfeb (Tfeb Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pC-sleeping beauty

Plasmid

#65487

Purpose

HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

Depositor

Guillaume Filion

Insert

sleeping beauty transposase

Expression

Insect

Promoter

HSP

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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p101_CRISPRai_PiggyBac

Plasmid

#213777

Purpose

Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

VPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator

Use

CRISPR

Promoter

EF1a (EF-1-alpha intron a) and Tet_On_TRE

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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