We narrowed to 6,478 results for: sas

1. pTarget_priming_VA

   Plasmid

   #186455

   Purpose

   pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget_PS_VB

   Plasmid

   #186456

   Purpose

   pTarget containing protospacer for AaCas12b targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH4143

   Plasmid

   #191531

   Purpose

   Punc-17-Cre unc-54 3'UTR C.elegans for Cre/Lox

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   no taq

   Expression

   Mutation

   Promoter

   Punc-17

   Available since

   Dec. 13, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJH3137

   Plasmid

   #179617

   Purpose

   Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   GCaMP6
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Punc-4

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJ414-PCY1:S495A

   Plasmid

   #98151

   Purpose

   Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:S495A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed serine 495 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ414-PCY1:W603A

   Plasmid

   #98153

   Purpose

   Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:W603A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tryptophan 603 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ414-PCY1:H695Q

   Plasmid

   #98154

   Purpose

   Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695Q
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to glutamine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ414-PCY1:H695A

   Plasmid

   #98155

   Purpose

   Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ414-PCY1:Y696G

   Plasmid

   #98156

   Purpose

   Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:Y696G
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tyrosine 696 to glycine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-FLAG-Tapasin-TAPBPR 22-35

    Plasmid

    #153478

    Purpose

    Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

    Depositor

    Erik Procko

    Article

    McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

    Insert

    TAPBP (TAPBP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS

    Plasmid

    #153471

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with tapasin CT deltaALAS

    Depositor

    Erik Procko

    Article

    McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C terminal tail of tapasin, deletion of amino aci…

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-FLAG-TAPBPR-tapasinCT delta24-36

    Plasmid

    #153472

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with tapasin CT delta24-36

    Depositor

    Erik Procko

    Article

    McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C terminal tail of tapasin, deletion of amino aci…

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EC71102

    Plasmid

    #154065

    Purpose

    Level 0 Golden Gate vector; Unmodifed CRE

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    CRE
    

    Use

    Synthetic Biology; Standard cloning vector used b…

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-gata2a

    Plasmid

    #132977

    Purpose

    full-length of gata2a without stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    gata2a (gata2a Zebrafish)
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDR-nfatc1_sgRNA2

    Plasmid

    #132978

    Purpose

    nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nfatc1 sgRNA2 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDR-itga9_sgRNA1

    Plasmid

    #132979

    Purpose

    itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA1 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDR-itga9_sgRNA4

    Plasmid

    #132980

    Purpose

    itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA4 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDR-itga9_sgRNA7

    Plasmid

    #132981

    Purpose

    itga9 sgRNA targeting to "5'-CCCTCCAGCCTTCCATATG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA7 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB12_TtNAM-B1

    Plasmid

    #124573

    Purpose

    Expresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-B1
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    35s

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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