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pSJX024

Plasmid

#232327

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al bioRxiv 2024.12.02.626314

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and cat

Expression

Bacterial

Promoter

PJ23119 and Pxyl

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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ChlorON-2

Plasmid

#193478

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-2

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMP116

Plasmid

#207918

Purpose

GreenBraid destination vector pGBZ1 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-HsTRBP

Plasmid

#41096

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

TRBP (TARBP2 Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMP117

Plasmid

#207920

Purpose

GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKD013

Plasmid

#207931

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTX171

Plasmid

#89258

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAC14

Plasmid

#197408

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pADtet-His6-MscL

Plasmid

#83373

Purpose

Expresses wild type MscL in mammalian cells

Depositor

Allen Liu

Article

Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

Insert

Mechanosensitive channel of large conductance (mscL E. coli)

Use

Adenoviral

Tags

His6 tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-Ace2_D92N_E199V-mRuby3-ST

Plasmid

#129262

Purpose

Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKD016

Plasmid

#207932

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

pZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMP119

Plasmid

#207949

Purpose

GreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pKE014

Plasmid

#207908

Purpose

GG entry for zCas9i expression

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

SpCas9_introns_2xNLS-Pea3A(T) term

Use

CRISPR

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMP107

Plasmid

#207925

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAM750

Plasmid

#207889

Purpose

Level 0 for A3 module (5' TCCC-3' AACA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM749

Plasmid

#207888

Purpose

Level 0 for AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge