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Plasmid#166952PurposeLentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoterDepositorInsertKIF5A (Kif5a Mouse)
UseLentiviralTagsHAExpressionMutationPromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE
Plasmid#235307PurposeLentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)DepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE
Plasmid#235303PurposeLentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE
Plasmid#235304PurposeLentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE
Plasmid#235308PurposeLentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)DepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE
Plasmid#235305PurposeLentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE
Plasmid#235306PurposeLentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)DepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-HRAS(G12V)-IRES-SNAP-p53DD
Plasmid#233200PurposeRetroviral expression of HRAS(G12V) and SNAP-p53DDDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233198PurposeRetroviral expression of SNAP-p53DD and HRAS(G12V)DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233201PurposeGateway entry vector for SNAP-p53DD and HRAS(G12V)DepositorUseOtherTagsExpressionMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUW-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233206PurposeLentiviral dox-inducible expression of SNAP-p53DD and HRAS(G12V)DepositorUseLentiviralTagsExpressionMammalianMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HRAS(G12V)-IRES-SNAP-p53DD
Plasmid#233199PurposeGateway entry vector for HRAS(G12V) and SNAP-p53DDDepositorUseOtherTagsExpressionMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HRAS(G12V)-IRES-p53DD
Plasmid#233186PurposeGateway entry vector for HRAS(G12V) and p53DD (RIDD)DepositorUseOtherTagsExpressionMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53DD-IRES-HRAS(G12V)
Plasmid#233187PurposeRetroviral expression of p53DD and HRAS(G12V) (DDIR)DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p53DD-IRES-HRAS(G12V)
Plasmid#233188PurposeGateway entry vector for p53DD and HRAS(G12V) (DDIR)DepositorUseOtherTagsExpressionMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-NIL
Plasmid#233154PurposeRetroviral expression of Ngn2, Isl1, and Lhx3 for motor neuron reprogrammingDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-YTHDF2-Malat1
Plasmid#216858PurposeCIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-Malat1
Plasmid#216860PurposeCIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-Malat1 gRNA
UseLentiviralTagsExpressionMammalianMutationPromoterSyn1, U6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
Plasmid#184563PurposeCMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines. Codon-opti…PromoterCMVAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-KIF5AR280H-HA-IRES-Puro
Plasmid#166953PurposeLentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoterDepositorInsertKIF5A-R280H (Kif5a Mouse)
UseLentiviralTagsHAExpressionMutationKIF5A-R280HPromotereF1aAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR Poison Exon Knockout Library
Pooled Library#138084PurposeThe human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-luc
Plasmid#129775PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferaseDepositorInserthuman MYC (MYC Human)
UseTagsluciferaseExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LNI (with mNgn2x3HA)
Plasmid#233195PurposeRetroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUW-LNI
Plasmid#233205PurposeLentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogrammingDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-LNI-bGH
Plasmid#233203PurposeAAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogrammingDepositorUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH
Plasmid#233204PurposeAAV expression of SNAP-p53DD and HRAS(G12V)DepositorUseAAVTagsExpressionMammalianMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-NIL (with mNgn2x3HA)
Plasmid#233192PurposeRetroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-NLI (with mNgn2x3HA)
Plasmid#233193PurposeRetroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LIN (with mNgn2x3HA)
Plasmid#233194PurposeRetroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ILN (with mNgn2x3HA)
Plasmid#233196PurposeRetroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-INL (with mNgn2x3HA)
Plasmid#233197PurposeRetroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CHyMErA LbCas12a Nuclease Optimization hgRNA Library
Pooled Library#209734PurposeCHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHyMErA AsCas12a Nuclease Optimization hgRNA Library
Pooled Library#209735PurposeCHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different AsCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
UseTagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CHyMErA Large-Scale Exon Deletion hgRNA Library
Pooled Library#209736PurposeCHyMErA Exon Deletion hgRNA Library allows identification of frame-preserving exons that affect cell fitness in human cells using the CHyMErA screening platform.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Human genome-wide lentiviral CRISPR gRNA library version 1
Pooled Library#67989PurposeHuman genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse genome-wide lentiviral CRISPR gRNA library version 2
Pooled Library#67988PurposeMouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS_101
Plasmid#160541PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_107
Plasmid#160650PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2-DHFR
Plasmid#163759PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFRDepositorInsertb2-DHFR (Dhfr Budding Yeast, Mouse)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Gaq-LSS-SGFP2
Plasmid#112951PurposeGalphaq tagged with LSS-SGFP2DepositorInsertGalphaq
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQFKan
Plasmid#32321DepositorInsertQF Kan
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMB52/Turkey_34_374
Plasmid#40545DepositorInsertLRRK2
UseBaculovirusTags6xHisExpressionMutationWTPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTV
Plasmid#131590PurposePlasmid for the targeting of chloramphenicol geneDepositorInsertNeomycin resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-SiT-N-15
Plasmid#54255PurposeLocalization: Golgi TGN, Excitation: 487, Emission: 509DepositorInsertSiT (ST6GAL1 Human)
UseTagsmEmeraldExpressionMammalianMutationaa 1-45 of SiTPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3EYE
Plasmid#234176PurposeHeterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coliDepositorInsert3EYE
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits