We narrowed to 2,475 results for: T2A
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Plasmid#195537PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)
Plasmid#176144PurposeEGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Gly60 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)
Plasmid#176143PurposeEGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Arg65 to Ala, and a mutation in the P…PromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)
Plasmid#176141PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutation in Asp256 to Ala, a…PromoterEF1AAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10
Plasmid#172917PurposeLexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7b-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10
Plasmid#172920PurposeLexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7s-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10
Plasmid#172919PurposeLexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7f-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161926PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertrTetR(SE-G72P)
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176272PurposeExpression of non-targeting sgRNA of CasMINIDepositorInsertCasMINI non-targeting sgRNA (sgLacZ) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176273PurposeExpression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.DepositorInsertCasMINI sgRNA (sgTet) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
Plasmid#187341PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE
Plasmid#188758PurposeLentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertVP64
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218910PurposePiggyBac transpositionDepositorInsertV5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
ExpressionMammalianMutation2363G>C and 4155C>T and 4729T>G and 4731…Available SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)
Plasmid#140226PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistanceDepositorInsertLuciferase + crIFNg
ExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140230PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFPDepositorInsertLuciferase + crIFNg (reverse orientation)
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone)
Plasmid#238188PurposeLentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668
Plasmid#238174PurposeLentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)
Plasmid#238189PurposeLentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666
Plasmid#238172PurposeLentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only