We narrowed to 6,912 results for: dna plasmids

1. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

   Plasmid

   #127332

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

   Plasmid

   #168102

   Purpose

   HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nEPEA-(EAAAK)3-OGA(544-706)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

   Plasmid

   #225588

   Purpose

   Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2(S54L+T127A)-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225584

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T21R-F8.2 (S54L+T127A)

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

   Plasmid

   #205162

   Purpose

   express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   scFv-GCN4-HAtag-msfGFP(V206K)-GBI
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

   Plasmid

   #238007

   Purpose

   300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

   Depositor

   Simon Powell, Agnel Sfeir

   Article

   Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

   Insert

   AAVS1 (AAVS1, PPP1R12C Human)
   

   Use

   Rna donor to study rt-dsbr

   Expression

   Mammalian

   Mutation

   3bp insertion signature (ATC) at Cas9 cut site in…

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Expression

   Mammalian

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182685

   Purpose

   Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

   Plasmid

   #47542

   Purpose

   engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   Glutamate-gated chloride channels
   

   Expression

   Mammalian

   Mutation

   retention motif in ER (starting at R340) changed …

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid

    #182686

    Purpose

    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Expression

    Mammalian

    Promoter

    NA

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CelTag Plasmid

    Plasmid

    #66562

    Purpose

    Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

    Depositor

    David Engelke

    Article

    Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

    Insert

    CelTag
    

    Expression

    Bacterial

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_ancCas9d_Plasmid

    Plasmid

    #239444

    Purpose

    Expression plasmid codon-optimized for E. coli

    Depositor

    David Taylor

    Article

    Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

    Insert

    ancCas9d
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_Cas9d_Plasmid

    Plasmid

    #239445

    Purpose

    Expression plasmid codon-optimized for E. coli

    Depositor

    David Taylor

    Article

    Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

    Insert

    Cas9d
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CJ8421_04975 Rptr2 recording plasmid

    Plasmid

    #194211

    Purpose

    CJ8421_04975 Rptr2 targeted DNA sequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 Rptr2 DNA target
    

    Expression

    Bacterial

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7 promoter + terminators reporter plasmid

    Plasmid

    #156456

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid

    #200905

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (pTarget NGS)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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