We narrowed to 775 results for: NEB

1. JD134

   Plasmid

   #229490

   Purpose

   Adenoviral E4 gene

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E4
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   wt promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP H2B SL2

   Plasmid

   #227718

   Purpose

   Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   GFP H2B SL2 (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T2A GFP H2B

   Plasmid

   #227722

   Purpose

   Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   (nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   T2A 3xNLS GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SL2 GFP H2B

   Plasmid

   #227717

   Purpose

   Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   SL2 GFP H2B (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MDH1_KW

   Plasmid

   #224145

   Purpose

   IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

   Depositor

   Katherine Walstrom

   Article

   Thomas et al Biochim Biophys Acta Proteins Proteom. 2

   Insert

   MDH-1 (F46E10.10 Nematode)
   

   Use

   Tags

   Ala-intein-CBD

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Strep-TGT-His

   Plasmid

   #206495

   Purpose

   Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

   Insert

   E. coli tRNA Guanine Transglycosylase
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTYB2-eIF4B-A130C

   Plasmid

   #37227

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   TIF3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A130C

   Promoter

   T7

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Avr2-GFP pZK538

   Plasmid

   #118011

   Purpose

   expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

   Depositor

   FLW Takken

   Article

   et al Bio-protocol 8(24): e3114.

   Inserts

   dspAvr2
   mCherry
   

   Use

   Tags

   EGFP and HDEL

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JD94

    Plasmid

    #229479

    Purpose

    CMV promoter driving expression of adenoviral E2A gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JD80

    Plasmid

    #229480

    Purpose

    Adenoviral VA RNA I gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SNAPtag-LacI

    Plasmid

    #183922

    Purpose

    Lac repressor labeled with SNAPtag; can be used to label lacO sites

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    SNAPtag-LacI (lacI Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV/MASC_(GLuc)_TOP1

    Plasmid

    #68439

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

18. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pJK240

    Plasmid

    #71127

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    GyrA intein and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge