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Plasmid#21193DepositorUseRetroviralTagsExpressionMutationMek1 R4F fused to ER*, constitutive activePromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only
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pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSbi-pur-KSR1-CA3-EGFP (C-KSR)
Plasmid#217768PurposeFluorescent reporter for ceramide (for stable mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro
Plasmid#44195DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsFrb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PIK3R1-1/1
Plasmid#91274PurposeProtein expression and purification of human SH3 domain construct PIK3R1-1/1DepositorInsertPIK3R1-1/1 (PIK3R1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF6-1/1
Plasmid#91515PurposeProtein expression and purification of human SH3 domain construct ARHGEF6-1/1DepositorInsertARHGEF6-1/1 (ARHGEF6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP2-1/1
Plasmid#91504PurposeProtein expression and purification of human SH3 domain construct ASAP2-1/1DepositorInsertASAP2-1/1 (ASAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA1
Plasmid#74446PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 1DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-mSNCA-NE
Plasmid#102364PurposeLentiviral overexpression of PA-mCherry synuclein alpha fusionDepositorInsertsUseLentiviralTagsNEExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_A
Plasmid#74374PurposegRNA_A to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIN-hSNCA-NE
Plasmid#102366PurposeLentiviral overexpression of synuclein alphaDepositorInsertsynuclein alpha (SNCA Human)
UseLentiviralTagsNEExpressionMammalianMutationA silent mutation (333bp downstream of ATG) was i…PromoterEF-1aAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
a4 ecto pcDNA3.1 hygro(-)
Plasmid#113406Purposeexpression of human alpha 4 ectodomain with purification tags and C-terminal claspDepositorInserthuman alpha 4 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-1/3
Plasmid#91472PurposeProtein expression and purification of human SH3 domain construct NCK1-1/3DepositorInsertNCK1-1/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)
Plasmid#99343PurposeBacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TADDepositorInsertsUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s (AAV8)
Viral Prep#105714-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-fDIO-GCaMP6s (#105714). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6s plasmid DNA. EF1a-driven, Flp recombinase-dependent expression of GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animalsDepositorPromoterEf1a, Human elongation factor-1 alphaTagsNoneAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only