We narrowed to 623 results for: SPEN;

1. pTK-EdnrbE2-mCherry

   Plasmid

   #127772

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVC-Ds-DrU6a:sgRNA-Ds

   Plasmid

   #119069

   Purpose

   To clone sgRNA spacer sequence for microinjections in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK-RetE1-Citrine

   Plasmid

   #127779

   Purpose

   Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   RetE1
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTK-EdnrbE2-Cerulean

   Plasmid

   #127771

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTK-EdnrbE1-Citrine

   Plasmid

   #127773

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE1
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-Sox3gRNA-T2

   Plasmid

   #127778

   Purpose

   Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox3gRNAT2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK_Cerulean_Nanotag_92_positive_Control

   Plasmid

   #130573

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   FoxD3_cranial_neural_crest_enhancer_NC4
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTK_Cerulean_Nanotag_91_positive_Control

   Plasmid

   #130572

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   FoxD3_cranial_neural_crest_enhancer_NC3
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

    Plasmid

    #119070

    Purpose

    Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

    Plasmid

    #119071

    Purpose

    Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

    Plasmid

    #119072

    Purpose

    Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled3-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

    Plasmid

    #119073

    Purpose

    Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled4-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSH1/M-Fv-Casp3-E

    Plasmid

    #15271

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36)-Caspase3 (CASP3 Human)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FKBP12(V36) F36->V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSH1/Mf-del-Akt-E

    Plasmid

    #15288

    Depositor

    David Spencer

    Article

    Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

    Insert

    delPH-Akt (Akt1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain Fy…

    Expression

    Mammalian

    Mutation

    Truncated PH (residues 1-99)

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge