We narrowed to 1,807 results for: Tef

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

TEF

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(504)

Plasmid

#44372

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p1977 (pUC19-PTDH3-PTEF1)

Plasmid

#78230

Purpose

Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Bidirectional promoters pTDH3-pTEF1

Expression

Bacterial

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pPotef-LN22*-3xGNLS-cbx

Plasmid

#86780

Purpose

Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

Depositor

Michael Feldbrügge

Article

Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

Insert

LN*

Use

Ustilago maydis expression

Tags

Gfp

Expression

Bacterial

Promoter

Potef

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(1004)

Plasmid

#44365

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(1004)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDSM-ab-Psptef1-NAT-Ttip1

Plasmid

#127747

Purpose

Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

nourseothricin N-acetyltransferase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptef1

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p416TEF1 Cystector (Cysteine biosensor)

Plasmid

#250654

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316-pTEF-Sc_IntAct-tCYC

Plasmid

#248980

Purpose

Low-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)

Depositor

Saravanan Palani

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Sc_IntAct

Tags

ALFA tag

Expression

Yeast

Promoter

TEF

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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