We narrowed to 1,947 results for: biotin

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C321.ΔA.exp

Bacterial strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Publication

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial resistance

Bleocin (Zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles
pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Publication

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Publication

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Publication

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Tags

FLAG and Signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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C321.ΔA

Bacterial strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Publication

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles
pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Publication

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Tags

FLAG and Signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-NLS-BirA-2a-mCherry

Plasmid

#79886

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-MCS-NLS-BirA-2A-mCherry_Ras

Plasmid

#80059

Purpose

MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-NLS-BirA-2a-mCherry

Plasmid

#79886

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-MCS-NLS-BirA-2A-mCherry_Ras

Plasmid

#80059

Purpose

MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMTB2-NLS-BirA-2A-mCherry_Ras

Plasmid

#80067

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMTB2-NLS-BirA-2A-mCherry_Ras

Plasmid

#80067

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMT-myl7-NLS-BirA-2A-mCherry_Ras

Plasmid

#80061

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

Plasmid

#30184

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMT-myl7-NLS-BirA-2A-mCherry_Ras

Plasmid

#80061

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-zic2a-NLS-BirA-2A-mCherry_Ras

Plasmid

#80065

Purpose

Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Publication

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Zic2a enhancer driving c-fos promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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