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pETSUMO2_Bacteroidetes-bGSDM

Plasmid

#212124

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Bacteroidetes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Bacteroidetes-bGSDM

Plasmid

#212124

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Bacteroidetes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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mTurquoise-CIDEB-C-18

Plasmid

#55543

Purpose

Localization: , Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

CIDEB (CIDEB Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTurquoise-CIDEB-C-18

Plasmid

#55543

Purpose

Localization: , Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

CIDEB (CIDEB Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
lentiGuideFB-Puro-B

Plasmid

#192507

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

bU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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lentiGuideFB-Puro-B

Plasmid

#192507

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

bU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide-Puro-FE

Plasmid

#248560

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiGuide-Puro-FE

Plasmid

#248560

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pGuide-AVIL

Plasmid

#246656

Purpose

Guide RNA for human AVIL

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

AVIL guide RNA (AVIL Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-AVIL

Plasmid

#246656

Purpose

Guide RNA for human AVIL

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

AVIL guide RNA (AVIL Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-NTRK1

Plasmid

#246657

Purpose

Guide RNA for human NTRK1

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

NTRK1 guide RNA (NTRK1 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-MAFA

Plasmid

#246658

Purpose

Guide RNA for human MAFA

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

MAFA guide RNA (MAFA Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-RUNX3

Plasmid

#246659

Purpose

Guide RNA for human RUNX3

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

RUNX3 guide RNA (RUNX3 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-NTRK1

Plasmid

#246657

Purpose

Guide RNA for human NTRK1

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

NTRK1 guide RNA (NTRK1 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-MAFA

Plasmid

#246658

Purpose

Guide RNA for human MAFA

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

MAFA guide RNA (MAFA Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGuide-RUNX3

Plasmid

#246659

Purpose

Guide RNA for human RUNX3

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

RUNX3 guide RNA (RUNX3 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge