We narrowed to 341 results for: split gfp

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140230

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg (reverse orientation)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBa-LSS-GFP-LDLR wt

Plasmid

#98184

Purpose

Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

Depositor

Gary Banker, Marvin Bentley

Article

Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

Insert

LDLR (LDLR Human)

Tags

GFP and LDLR signal sequence, N-term of GFP so GF…

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta Actin

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Article

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Plasmid

#165030

Purpose

Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMRBAD-Z-CGFP

Plasmid

#52735

Purpose

L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

Z+CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

Plasmid

#216382

Purpose

Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

6xHis-3C-splitGFP11

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-link-NGFP

Plasmid

#52732

Purpose

IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-NGFP

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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PB-GFP11x7-HygR

Plasmid

#223676

Purpose

To check the split GFP signals in mitochondria (2)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP11x7

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NGFP

Plasmid

#52734

Purpose

IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

NGFP+Z-fusion

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pDAS12342_PEAR-GFP_2in1

Plasmid

#177184

Purpose

PEAR-GFP plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-10nmSAH-GFP(1-10)

Plasmid

#61660

Purpose

10nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-10nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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