We narrowed to 3,269 results for: ALC

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GlycophorinE-CD4d3+4-bio

Plasmid

#73095

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin E (GYPE Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FGF10-luciferase

Plasmid

#24994

Depositor

Benoit Bruneau

Article

Agarwal et al Development. 2003 Feb . 130(3):623-33.

Insert

Fgf10 promoter (Fgf10 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Changed aspartic acid 53 to alanine (D53A)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_Neo_FKBPF36V_mScarlet

Plasmid

#163886

Purpose

Acute Depletion of P300-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFlox c-CTCF FKBPF36V_EYFP_p2aBsd

Plasmid

#163870

Purpose

Acute Depletion of CTCF-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Ctcf (Ctcf Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-RPB1_BSD_FKBPF36V_EYFP

Plasmid

#163884

Purpose

Acute Depletion of RBP1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rbp1 (Polr2a Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ER-BSCaM2

Plasmid

#64855

Purpose

Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-BSCaM2

Tags

CFP, N-terminal 27 amino acids from CYP450, and Y…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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PGRMC2-CD4d3+4-bio

Plasmid

#73112

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PGRMC2 (PGRMC2 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn4

Plasmid

#164960

Purpose

Zebrafish kcnn4 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn4 (kcnn4 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLpA-JNK1

Plasmid

#51945

Purpose

Adenoviral vector for JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

JNK1 (Mapk8 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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pGBT-R16

Plasmid

#31827

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

mini Tol2 ITR

Use

Cre/Lox; Gene trap

Tags

mRFP

Mutation

mRFP lacks start codon

Promoter

beta Actin

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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IBMc071

Plasmid

#161943

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only