We narrowed to 10,295 results for: ESP

1. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

   Plasmid

   #229814

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Cre/Lox; Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

   Plasmid

   #229826

   Purpose

   Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

   Plasmid

   #229841

   Purpose

   A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-H2B-mCerulean-2xStop-loxP
   

   Use

   Cre/Lox; Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

   Plasmid

   #224496

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

   Plasmid

   #170193

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   E484K

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

   Plasmid

   #170194

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   E484K

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

   Plasmid

   #170189

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-GCaMP5G

   Plasmid

   #31788

   Purpose

   a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

   Depositor

   Loren Looger, Douglas Kim, GENIE Project

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   GCaMP5G
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

    Plasmid

    #159679

    Purpose

    Mammalian protein expression of KRAS4b-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.KRAS4b (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R713-M88-303: CMV51p> Halotag-Hs.HRAS

    Plasmid

    #159681

    Purpose

    Mammalian protein expression of HRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.HRAS (HRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R713-M91-303: CMV51p> Halotag-Hs.NRAS

    Plasmid

    #159682

    Purpose

    Mammalian protein expression of NRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.NRAS (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

    Plasmid

    #159696

    Purpose

    E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
    

    Use

    Tags

    His6-Halotag-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 18065-M03-412

    Plasmid

    #225666

    Purpose

    Lentivirus protein expression. Control plasmid.

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    WPRE-SV40p and mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

    Plasmid

    #159698

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

    Plasmid

    #159680

    Purpose

    Mammalian protein expression of KRAS4a-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.KRAS4a (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R725-X11-566: His6-MBP-tev-Hs.LGALS3

    Plasmid

    #159699

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.LGALS3

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

    Plasmid

    #159687

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Insert

    His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

    Plasmid

    #159700

    Purpose

    E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge