We narrowed to 1,917 results for: FRT

1. pGC225

   Plasmid

   #19636

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGC97

   Plasmid

   #19629

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGC238

   Plasmid

   #19631

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)

   Use

   Flp/frt, destination vector

   Tags

   4xNLS

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGC160

   Plasmid

   #19632

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDonorTp-fugfp

   Plasmid

   #211790

   Purpose

   pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   fuGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLN

   Plasmid

   #40029

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   FRT-loxP
   Neo
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   None and SV40

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGC162

   Plasmid

   #19633

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGC183

   Plasmid

   #19630

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
   

   Use

   Flp/frt, destination vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pADH99

   Plasmid

   #90979

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   US_HIS1
   FRT
   pENO1
   C. albicans Cas9
   pMAL2
   FLP
   NAT 1 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pADH100

    Plasmid

    #90980

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pADH147

    Plasmid

    #90991

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-fDIO-stGtACR2-FusionRed

    Plasmid

    #217975

    Purpose

    AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

    Depositor

    Edward Callaway

    Article

    Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

    Insert

    stGtACR2-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-CMV

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPYL-ABI

    Plasmid

    #233650

    Purpose

    Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

    Depositor

    Richard Wombacher

    Article

    Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

    Insert

    TOMM20-mCherry-PYL-P2A-eGFP-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGK plasmid

    Plasmid

    #169744

    Purpose

    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEXP(V5-APEX2-NES)

    Plasmid

    #107596

    Purpose

    Expresses V5-APEX2-NES in mammalian cells

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Insert

    APEX2-NES
    

    Use

    Frt site to insert into hek293 t-rex genome

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Dox inducible

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FR_HNF6

    Plasmid

    #31099

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF6 (ONECUT1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge