We narrowed to 4,435 results for: Gca

1. ANAPC1 A2.3 gRNA

   Plasmid

   #90529

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ANAPC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ANAPC1 (Guide Designation A2.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPneogRNA241510+MT

   Plasmid

   #84292

   Purpose

   Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
   

   Use

   CRISPR; Leishmania donovani

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCascade-IS2

   Plasmid

   #140625

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJG488

   Plasmid

   #91196

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nTaCas9_H840A+gUbi8+gUbi1+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15416-BEAR-mScarlet-target-BFP

   Plasmid

   #162996

   Purpose

   plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   sgRNA targeting the BEAR-mScarlet plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

   Plasmid

   #138985

   Purpose

   IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

   Plasmid

   #138984

   Purpose

   IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRB (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-puro-mU6-CNPshRNA1

   Plasmid

   #126611

   Purpose

   Expresses shRNA against human CNP under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP ShRNA-1 (CNP Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-shEsr1

    Plasmid

    #120720

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse U6

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. WPXL LEFTY2 gRNA

    Plasmid

    #101924

    Purpose

    LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    LEFTY2 enhancer targeting gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET a sfMatryoshCaMP6s

    Plasmid

    #100020

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PPP2R5D B12.5 gRNA

    Plasmid

    #90854

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP2R5D

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP2R5D (Guide Designation B12.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS19

    Plasmid

    #158788

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    psra-6::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS16

    Plasmid

    #158786

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Pntc-1::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458_KLF9_2

    Plasmid

    #86347

    Purpose

    Encodes gRNA for 3' target of human KLF9

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF9 (KLF9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. BUB3 A10.1 gRNA

    Plasmid

    #90557

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB3 (Guide Designation A10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pk335-CARGO-3mer-35kb-DSF

    Plasmid

    #227492

    Purpose

    3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 35kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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