We narrowed to 8,983 results for: ef1;

1. pCDH-CAG-IntCCCre-EF1a-Cerulean

   Plasmid

   #160508

   Purpose

   Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

   Plasmid

   #69582

   Purpose

   Expresses p53 tagged with mKate2 and split N-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - N term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1α-squirrel-monkey-CHMP3(155)-HA

   Plasmid

   #154173

   Purpose

   expresses squirrel monkey CHMP3(155) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3(155)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 155

   Promoter

   EF1-alpha

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-RoninDHSA-FLAG-IRES-Neo

   Plasmid

   #28021

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DHSA mutation (Y246A) of the previously defined H…

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF1-MCS2-EF1-Puro-RGS7

   Plasmid

   #113728

   Purpose

   Express RGS7 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-tetR-T2A-tSapphire

   Plasmid

   #176312

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   T-sapphire
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-CCre-MBP1-WPRE

   Plasmid

   #193917

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP1
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-NCre-MBP6-WPRE

   Plasmid

   #193916

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP6
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-myc-LRH1(ES)-IRES-neo

    Plasmid

    #28092

    Depositor

    Austin Cooney

    Article

    Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

    Insert

    Lrh-1 (Nr5a2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pEF1α-owl-monkey-retroCHMP3(148)-HA

    Plasmid

    #154252

    Purpose

    expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of retroCHMP3 at amino acid…

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-EF1a ANXA11-mEmerald R346C

    Plasmid

    #164213

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R346C

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R235Q

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

    Plasmid

    #117135

    Purpose

    Expression vector for Nkx2-5 and gRNA

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #128588

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-KIF5A-HA-IRES-Puro

    Plasmid

    #166952

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

    Plasmid

    #171689

    Purpose

    Flp-dependent expression of jGCaMP7b

    Depositor

    Mark Huebener, Tobias Bonhoeffer, Tobias Rose

    Article

    Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

    Insert

    GCaMP7b
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

    Plasmid

    #166412

    Purpose

    express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 K90E (POT1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    EF-1a

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid

    #121806

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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