We narrowed to 76,996 results for: rest

1. pX459-HypaCas9-ANLN_sgRNA

   Plasmid

   #183874

   Purpose

   pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1037 pGL3 FasL promoter

   Plasmid

   #9028

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

3. Beta2-AR-TYY

   Plasmid

   #32694

   Depositor

   Robert Lefkowitz

   Article

   Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

   Insert

   β2AR (ADRB2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T68F, Y132G Y219A

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pX459-HypaCas9-ECT2_sgRNA

   Plasmid

   #183873

   Purpose

   pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA Myc-mMEKK2

   Plasmid

   #44158

   Depositor

   Xin Lin

   Article

   Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

   Insert

   Mekk2 (Map3k2 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-sgRELA

   Plasmid

   #83944

   Purpose

   Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgRELA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Myc-huMEKK3 (K391A)

   Plasmid

   #44157

   Depositor

   Xin Lin

   Article

   Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

   Insert

   MEKK3 (K391A) (MAP3K3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K391A, kinase-dead mutant

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. E12-cTAP

   Plasmid

   #20916

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   E12 cTAP (Tcf3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TAP tag is fused to the 3' end of the E12 ge…

   Available Since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

9. 1129 pcDNA3 GFP FKHR H215R AAA

   Plasmid

   #9034

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pBV-Luc/Del-6

    Plasmid

    #14969

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-109/+334) (MYC Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -109/+334 (relative to the P2 transcription initi…

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available Since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 20XUAS IVS Jaws mCherry_tr

    Plasmid

    #111549

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXB7 (human) pCMV

    Plasmid

    #8539

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. HOXB4 (human) FLAG MSCV

    Plasmid

    #8528

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Missing N-terminal 14 amino acids; improved Ribos…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 10XUAS IVS Jaws mVenus_tr

    Plasmid

    #111553

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mVenus_tr
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2044

    Plasmid

    #79652

    Purpose

    ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple255-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 HS234Z Enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 13XLexAop2 IVS Jaws mCherry_tr

    Plasmid

    #111551

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FusXTBE-1397-C

    Plasmid

    #201398

    Purpose

    Expresses C terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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