161,271 results

1. TLCV2-DelGFP

   Plasmid

   #133302

   Purpose

   EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050

   Pooled Library

   #1000000131

   Purpose

   Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes

   Depositor

   David Root, John Doench

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.hSyn.eGFP.WPRE.bGH

   Plasmid

   #105539

   Purpose

   AAV expression of EGFP from hSyn promoter

   Depositor

   James M. Wilson

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVTNT-T7-KPNA2

   Plasmid

   #26678

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 . 11():63.

   Insert

   Karyopherin Alpha 2 (KPNA2 Human)
   

   Use

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-Prdm9CD

   Plasmid

   #235577

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Prdm9 (Prdm9 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (AAV1)

   Viral Prep

   #100842-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (#100842). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   CAG

   Tags

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

7. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-mito-dsRED2

   Plasmid

   #174541

   Purpose

   mitochondria targeted dsRED expressed off of EF1a promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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