161,271 results

1. pAAV-Ef1a-Con/Fon-GCaMP6F (AAV5)

   Viral Prep

   #137122-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pCH67 (crRNA expression vector)

   Plasmid

   #217337

   Purpose

   CROP-seq crRNA expression vector with GFP and puro marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Golgi-mEGFP

   Plasmid

   #182877

   Purpose

   Localization: Gal-T - Golgi Complex

   Depositor

   Salvatore Chiantia

   Article

   Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

   Insert

   Golgi-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NRCH-YE1-BE4max

   Plasmid

   #198549

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 . 11():63.

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-flag-hKDM4A-polyA

   Plasmid

   #101051

   Purpose

   in vitro transcription (T7 promoter)

   Depositor

   Yi Zhang

   Article

   Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

   Insert

   KDM4A (KDM4A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

   Plasmid

   #205703

   Purpose

   CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Use

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-LaminB1-10

    Plasmid

    #55069

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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