We narrowed to 2,260 results for: PAM

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pMXS-IRES-BLAST flag-CAD hCPS1 TL

Plasmid

#188150

Purpose

Expresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only
Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

Plasmid

#140001

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max SpCas9-NG with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-eGFP-SynaptoZip

Plasmid

#122523

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hPGK

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

Plasmid

#139998

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2382

Plasmid

#225638

Purpose

AAV plasmid with Ple384 (TH MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Sci Rep. 2026 Feb 16. doi: 10.1038/s4159

Insert

Ple384 (TH Human)

Use

AAV

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

Plasmid

#184631

Purpose

Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad65 (Gad2 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

Plasmid

#139992

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VRQR=D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-mCherry-1x miR30 SCR

Plasmid

#216393

Purpose

transfer plasmid for AAV vector production of mCherry and miR scramble (no target)

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR control

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLV.tetO.lmx1a

Plasmid

#234847

Purpose

3rd generation lentiviral vector, expresses Lmx1a under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Lmx1a (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

Plasmid

#139990

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VQR=D1135V/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

Plasmid

#139991

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VRER=D1135V/G1218R/R1335E/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

Plasmid

#139995

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

HF1=N497A/R661A/Q695A/Q926A

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

Plasmid

#209198

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Article

Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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