We narrowed to 165,694 results for: addgene

1. cTRE-PtenC124S

   Plasmid

   #135664

   Purpose

   Introduce Dox-inducible (TREa promoter) C124S-mutant Pten cDNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   Pten (Pten Mouse)
   

   Use

   Mouse Targeting

   Mutation

   C124S

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5J39 (TDRKH)

   Plasmid

   #79523

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD2 (TDRKH Human)
   

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   June 16, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. integrin beta6 Y762F, Y774F pBabe puro

   Plasmid

   #13598

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 Y762F Y774F (ITGB6 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Y762F Y774F

   Available Since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREST.1.0-gDNA

   Plasmid

   #113774

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST

   Depositor

   Kevin White

   Insert

   REST (REST Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p14-9VN9-3A

   Plasmid

   #50932

   Purpose

   Expresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.

   Depositor

   Katharina Strub

   Insert

   SRP14 and SRP9 (SRP14, SRP9 Human)
   

   Tags

   VN tag and flag tag

   Expression

   Mammalian

   Mutation

   Amino acids 60, 61 and 64 of SRP9 changed to alan…

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUK21-NotI

   Plasmid

   #51660

   Purpose

   Bacterial cloning vector based on pUK21 (Kan)

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVLINK-EF1alpha-5'-NRXN2

   Plasmid

   #244274

   Purpose

   5' AAVLINK plasmid for NRXN2 expression

   Depositor

   Zhonghua Lu

   Insert

   NRXN2 (NRXN2 Human)
   

   Use

   AAV

   Tags

   HA

   Promoter

   EF1alpha

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLFYa

   Plasmid

   #243803

   Purpose

   Dropout vector for genomic integration into yeast strain LFYa. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains the attP site for the serine recombinase BxB1.

   Depositor

   Sai Reddy

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLFYalpha

   Plasmid

   #243804

   Purpose

   Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.

   Depositor

   Sai Reddy

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVLINK-EF1alpha-5' NUP155

    Plasmid

    #236644

    Purpose

    5' AAVLINK plasmid for NUP155 expression

    Depositor

    Zhonghua Lu

    Insert

    NUP155 (NUP155 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVLINK-EF1alpha-5' PREX1

    Plasmid

    #236708

    Purpose

    5' AAVLINK plasmid for PREX1 expression

    Depositor

    Zhonghua Lu

    Insert

    PREX1 (PREX1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVLINK-EF1alpha-5' PRR12

    Plasmid

    #236676

    Purpose

    5' AAVLINK plasmid for PRR12 expression

    Depositor

    Zhonghua Lu

    Insert

    PRR12 (PRR12 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVLINK-EF1alpha-5' NINL

    Plasmid

    #236690

    Purpose

    5' AAVLINK plasmid for NINL expression

    Depositor

    Zhonghua Lu

    Insert

    NINL (NINL Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVLINK-EF1alpha-5' NEO1

    Plasmid

    #236700

    Purpose

    5' AAVLINK plasmid for NEO1 expression

    Depositor

    Zhonghua Lu

    Insert

    NEO1 (NEO1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195623

    Purpose

    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    EF1a (Mlu1/EcoR1)

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVLINK-EF1alpha-5' UBN2

    Plasmid

    #236678

    Purpose

    5' AAVLINK plasmid for UBN2 expression

    Depositor

    Zhonghua Lu

    Insert

    UBN2 (UBN2 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVLINK-EF1alpha-5' PHRF1

    Plasmid

    #236706

    Purpose

    5' AAVLINK plasmid for PHRF1 expression

    Depositor

    Zhonghua Lu

    Insert

    PHRF1 (PHRF1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2-ACTB-C4

    Plasmid

    #229849

    Purpose

    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB_EF1a_mNeon

    Plasmid

    #212653

    Purpose

    piggyBac vector to express mNeon

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    mNeon
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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