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We narrowed to 173,443 results for: addgene

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Showing: 1401 - 1420 of 173443 results

  1. FOR human NANOS3 TALEN

    Plasmid
    #59722
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. REV human NANOS3 TALEN

    Plasmid
    #59723
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  4. attB53-Pac-TRE-3xFlag-Neurog1-attB53

    Plasmid
    #183613
    Purpose
    RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    Neurog1 (Neurog1 Mouse)
    Use
    Rmce
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183613-plasmid-map-sequence-id-361602

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  8. TEVEp_pRK793

    Plasmid
    #247672
    Purpose
    pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.
    Depositor
    Carl Denard
    Article
    Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2
    Insert
    MBP-ENLYFES-7xHIS-TEVEp
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    S120R, D148R, T173A, N177K, M218I, S219P
    Available Since
    Feb. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    247672-plasmid-map-sequence-id-491563

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  9. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232931-plasmid-map-sequence-id-466490

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  16. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  18. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-ALFA

    Plasmid
    #242782
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-ALFA in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    ALFA
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242782-plasmid-map-sequence-id-486993

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  19. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-AU5

    Plasmid
    #242783
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-AU5 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    AU5
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242783-plasmid-map-sequence-id-486992

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  20. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-E2

    Plasmid
    #242784
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-E2 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    E2
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242784-plasmid-map-sequence-id-486990

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Showing: 1401 - 1420 of 173443 results