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We narrowed to 63,783 results for: cloning

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Showing: 1401 - 1420 of 63783 results

  2. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119749-plasmid-map-sequence-id-233993

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  4. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Publication
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Publication
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Promoter
    U6 promoter from W05B2.8
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  16. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid
    #132962
    Purpose
    Firefly-luciferase single-strand annealing assay cloning backbone
    Depositor
    Gang Bao
    Publication
    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly Luciferase
    Use
    CRISPR and Luciferase
    Expression
    Mammalian
    Mutation
    Stop sequence between luciferase gene
    Promoter
    CMV
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132962-plasmid-map-sequence-id-259217

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  17. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99698-plasmid-map-sequence-id-194602

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  20. pSBtet-Hyg

    Plasmid
    #60508
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60508-plasmid-map-sequence-id-97539

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Showing: 1401 - 1420 of 63783 results