We narrowed to 4,131 results for: p2a

1. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3

   Plasmid

   #172921

   Purpose

   For enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3

   Depositor

   GENIE Project

   Has Service

   AAV1 and AAV9

   Insert

   axon-jGCaMP8s-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain and P2A-mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DIO-CAG-hM4Di-P2A-dTomato

   Plasmid

   #202037

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

   Plasmid

   #214989

   Purpose

   Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

   Plasmid

   #154004

   Purpose

   Express CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-mCardinal-P2A-B19L Flp-in vector

   Plasmid

   #178519

   Purpose

   For making TRE-CB mouse line in Tigre locus

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCardinal-P2A-B19L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1

   Plasmid

   #126945

   Purpose

   Expression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

   Insert

   SomArchon-P2A-CoChR-Kv2.1
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73039

   Purpose

   Express Nluc, copGFP dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin + CMV enhancer and chicken bet…

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-p2A-CreERT2-FRT-kan-FRT

    Plasmid

    #108454

    Purpose

    This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    mCherry-p2a-CreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CAG-REV1-p2A-GFP-PuroR

    Plasmid

    #153001

    Purpose

    Overexpression of human REV1

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    REV1 (REV1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-mCherry-p2A-full length LIC1

    Plasmid

    #74601

    Purpose

    expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-NES-SspB-MCS-P2A-iLIDcaax

    Plasmid

    #173869

    Purpose

    Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-MCS-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

    Plasmid

    #129111

    Purpose

    Homologous recombination vector for excision reporter 1 in the CLYBL locus.

    Depositor

    Bruce Conklin

    Insert

    LSL, eGFP, NLS-RFP
    

    Use

    Cre/Lox and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFSW GAP43-jRGECO1a P2A Syp-Halo

    Plasmid

    #185565

    Purpose

    Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

    Insert

    GAP43-jRGECO1a P2A Syp-Halo
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CamKII-GFP-P2A-bReaches-minWPRE

    Plasmid

    #118276

    Purpose

    Expresses EGFP and bReaChES under a minimal CamKII Promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    EGFP
    bReaCHES
    

    Use

    AAV

    Tags

    P2A

    Expression

    Mutation

    Promoter

    CamK II

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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