Skip to main content
Addgene
Showing: 1401 - 1420 of 44345 results

  1. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

    Enlarge

  7. pUPD2 NtSPrbcS (GB2213)

    Plasmid
    #160589
    Purpose
    B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain
    Depositor
    Diego Orzaez
    Insert
    NtSPrbcS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160589-plasmid-map-sequence-id-322077

    Enlarge

  20. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid
    #70651
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1401 - 1420 of 44345 results