We narrowed to 10,294 results for: otos

Showing: 2131 - 2160 of 10294 results

Results per page:

Sort results by:

pMXs-LIN (with mNgn2x3HA)

Plasmid

#233194

Purpose

Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-Isl1-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ILN (with mNgn2x3HA)

Plasmid

#233196

Purpose

Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mLhx3-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-INL (with mNgn2x3HA)

Plasmid

#233197

Purpose

Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mNgn2x3HA-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

Plasmid

#192704

Purpose

Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Tags

Ig k-chain leader sequence, SNAPf-tag, and meGFPm…

Expression

Mammalian

Mutation

meGFPm: gluatmic acid 142 to asparagine and histi…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

Plasmid

#192784

Purpose

Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Tags

Ig k-chain leader sequence, SNAPf-tag, and mXFPm

Expression

Mammalian

Mutation

mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCM-CLYBL-hNIL

Plasmid

#105841

Purpose

Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMAL-CellTag-V1

Pooled Library

#115643

Purpose

Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Has Service

Concentrated Lentiviral Prep

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only
Mouse Metabolism Library

Pooled Library

#163966

Purpose

Knockout library targeting mouse metabolic genes.

Depositor

Alec Kimmelman

Article

Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Use

CRISPR and Lentiviral

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-V2

Pooled Library

#115644

Purpose

Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Has Service

Concentrated Lentiviral Prep

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-V3

Pooled Library

#115645

Purpose

Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Has Service

Concentrated Lentiviral Prep

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only
PB-GG-OMKSL-PGK-Puro

Plasmid

#102894

Purpose

PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-EEA-5g-OSK2M2L1-PGK-Puro

Plasmid

#102909

Purpose

PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GG-EBNA-OMKSL-PP

Plasmid

#102899

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p11-5’_10xN_PAM-site3 (RTW572)

Pooled Library

#160134

Purpose

Plasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
p11-3’_8xN_PAM-site1 (RTW554)

Pooled Library

#160132

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
p11-5’_10xN_PAM-site4 (RTW574)

Pooled Library

#160135

Purpose

Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
p11-3’_8xN_PAM-site2 (RTW555)

Pooled Library

#160133

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
pSMAL-CellTag-multi-v1 barcode library

Pooled Library

#206045

Purpose

The CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal tracking

Depositor

Samantha Morris

Article

Jindal et al Nat Biotechnol. 2023 Sep 25. doi: 10.103

Use

Lentiviral

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only
Human SLC KO Library

Pooled Library

#132552

Purpose

Knockout library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Article

Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
plIG-123_HA-GD2-28z_CAR_TF_Library

Pooled Library

#207478

Purpose

HA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-128_HA-GD2-28z_CAR_TFxTF_Library

Pooled Library

#207480

Purpose

HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-106_NY-ESO-1_TCR_SR_Library

Pooled Library

#207474

Purpose

NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-107_NY-ESO-1_TCR_TF_Library

Pooled Library

#207475

Purpose

NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-109_CD19-BBz_CAR_SR_Library

Pooled Library

#207476

Purpose

CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-110_CD19-BBz_CAR_TF_Library

Pooled Library

#207477

Purpose

CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-124_HA-GD2-28z_CAR_SR_Library

Pooled Library

#207479

Purpose

HA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cel

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-132_CD19-28z_CAR_TF_and_SR_Library

Pooled Library

#207481

Purpose

CD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
pGLU_112

Plasmid

#225793

Purpose

RS421 library variant 13926

Depositor

Nili Ostrov

Insert

13926_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLU_76

Plasmid

#225778

Purpose

RS421 library variant 13926

Depositor

Nili Ostrov

Insert

13926_[abr]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge