We narrowed to 7,368 results for: GAN

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pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUBC_V5_MS2-mVenus

Plasmid

#107606

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

Depositor

John Rinn

Publication

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

MS2-mVenus

Use

Synthetic Biology

Tags

V5 and mVenus

Mutation

MS2: V75E/A81G

Promoter

human UBC

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM75

Plasmid

#133649

Purpose

Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-ANP1-mCherry (ANP1 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Expression

Worm

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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1068 pGL3 FasL promoter (no SV40 prom)

Plasmid

#9029

Depositor

William Sellers

Publication

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Removed the SV40 promoter from pGL3

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Publication

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LGC-53

Plasmid

#86399

Purpose

Mammalian expression of codon-optimized dopamine receptor LGC-53

Depositor

Steven Mennerick

Publication

Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

Insert

LGC-53 (lgc-53 Synthetic, Nematode)

Expression

Mammalian

Mutation

Codon optimized for mammalian expression; Signal …

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Publication

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD4 galK-GT

Plasmid

#19183

Depositor

Al Fisher

Publication

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

galK (galK E.coli)

Use

Recombineering

Tags

50 nucleotides from N-term TAP, C-term TAP, and F…

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pOX + kqt3

Plasmid

#16138

Depositor

Larry Salkoff

Publication

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-3 Nematode)

Use

Xenopus oocyte expression

Mutation

lacks aa1-16 compared to GenBank reference AY5729…

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-WT-WLS-HA-IRES-GFP

Plasmid

#178061

Purpose

Expression of WLS-HA protein (WT)

Depositor

Joseph Gleeson

Publication

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-muGFP

Plasmid

#166727

Purpose

AAV, encodes monomeric ultrastable GFP (muGFP)

Depositor

Daniel Scott

Publication

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

AAV

Promoter

Chicken Beta Actin

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

Plasmid

#127355

Purpose

gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and mVenus

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSF11-wmTagBFP2

Plasmid

#184934

Purpose

Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2

Expression

Worm

Promoter

tag-168

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTXRE5-Z

Plasmid

#112509

Purpose

Reporter plasmid, responsive to activated Ah receptor/Arnt

Depositor

Charles Miller

Publication

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

RW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)

Expression

Yeast

Promoter

5 XREs+ minimal Cytochrome C

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-NHERF2

Plasmid

#28292

Depositor

Maria-Magdalena Georgescu

Publication

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Publication

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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