We narrowed to 1,670 results for: nif

1. SIV_Rev

   Plasmid

   #236244

   Purpose

   3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   SIV Rev
   

   Use

   Lentiviral

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Expression

   Mammalian

   Mutation

   R203M

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-N-WT-Hu1

   Plasmid

   #177937

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a and U6

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL023_Tub-rb_IgG_HC

   Plasmid

   #193620

   Purpose

   α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Tubulin scFv heavy variable + rabbit constant chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDL024_Tub-rb_IgG_LC

   Plasmid

   #193621

   Purpose

   α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Tubulin scFv light variable + rabbit constant chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. GFP-p58

   Plasmid

   #166942

   Purpose

   mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

   Insert

   p58 (Lman1 Rat)
   

   Tags

   GFP and chicken lysozyme signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Klf2-t2A-mCherry

   Plasmid

   #127539

   Purpose

   Expresses mouse Klf2 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   Klf2 (Klf2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-S202R

   Plasmid

   #177950

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S202R) (N )
   

   Expression

   Mammalian

   Mutation

   S202R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-UBC-hAAT-IRES-ZsGreen-W

    Plasmid

    #126702

    Purpose

    lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

    Depositor

    Darrell Kotton

    Article

    Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

    Insert

    Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
    

    Use

    Lentiviral

    Promoter

    UBC

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCerulean-VSVG

    Plasmid

    #11913

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Expression

    Mammalian

    Mutation

    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. CoV2-N-P199L

    Plasmid

    #177949

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P199L) (N )
    

    Expression

    Mammalian

    Mutation

    P199L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and EF1-a

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1365 LV EF1a-CD28 IRES-EGFP

    Plasmid

    #201921

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPS0382

    Plasmid

    #133232

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Expression

    Yeast

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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