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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,774 results for: venus

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Showing: 1421 - 1440 of 1774 results

  1. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112060-plasmid-map-sequence-id-216934

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  2. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid
    #49258
    Purpose
    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
    Depositor
    Douglas Green
    Publication
    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    caspase-2 (CASP2 Human)
    Tags
    flag and split Venus N
    Expression
    Mammalian
    Mutation
    C303S
    Promoter
    CMV
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid
    #49260
    Purpose
    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
    Depositor
    Douglas Green
    Publication
    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    caspase-2 CARD (CASP2 Human)
    Tags
    Flag and split Venus N
    Expression
    Mammalian
    Mutation
    delete aa 123-435
    Promoter
    CMV
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pBiFC-HA-hCaspase 2 prodomain-VC155

    Plasmid
    #49261
    Purpose
    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
    Depositor
    Douglas Green
    Publication
    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    caspase-2 prodomain (CASP2 Human)
    Tags
    HA and split Venus C
    Expression
    Mammalian
    Mutation
    delete aa 148-435
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  8. pBIFC-flag-hCaspase 2 prodomain-VN173

    Plasmid
    #49262
    Purpose
    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.
    Depositor
    Douglas Green
    Publication
    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    caspase-2 prodomain (CASP2 Human)
    Tags
    flag and split Venus N
    Expression
    Mammalian
    Mutation
    delete aa 148-435
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  10. pJW1507

    Plasmid
    #62361
    Purpose
    pFA6a -mVenus-BirA::HIS5
    Depositor
    Jonathan Weissman
    Publication
    Jan et al Science. 2014 Nov 7;346(6210):1257521. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    c-terminal mVenus-BirA tagging
    Use
    Yeast c-terminal tagging vector
    Tags
    mVenus-BirA
    Mutation
    L232H mutation compared to standard mVenus
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62361-plasmid-map-sequence-id-112196

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  11. pcDNA3.1(+)-ER-CaNARi

    Plasmid
    #64852
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNARi
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64852-plasmid-map-sequence-id-109677

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  12. pcDNA3.1(+)-PM-CaNARi

    Plasmid
    #64850
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-CaNARi
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64850-plasmid-map-sequence-id-109675

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  13. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Publication
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  16. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ICUE4 (RAPGEF3 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    181846-plasmid-map-sequence-id-364236

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  17. Epac-S-H326

    Plasmid
    #228242
    Purpose
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228242-plasmid-map-sequence-id-454214

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  18. Epac-S-H327

    Plasmid
    #228243
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228243-plasmid-map-sequence-id-451727

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  19. Epac-S-H328

    Plasmid
    #228244
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228244-plasmid-map-sequence-id-451838

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  20. Epac-S-H329

    Plasmid
    #228245
    Purpose
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228245-plasmid-map-sequence-id-451790

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Showing: 1421 - 1440 of 1774 results