We narrowed to 2,307 results for: pET28

1. pHis-hTG2

   Plasmid

   #100719

   Purpose

   Expression of human transglutaminase 2 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   silent mutation Arg580 (AGG--CGT) to enhance expr…

   Promoter

   T7

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHYP-NPC-10H

   Plasmid

   #162789

   Purpose

   SARS-CoV-2 Nucleocapsid Protein expression in E.coli

   Depositor

   Ivan Vorobiev

   Article

   Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

   Insert

   Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHis-hPim1

   Plasmid

   #100722

   Purpose

   Expression of human Pim1 kinase domain in E.coli

   Depositor

   Byung Il Lee

   Article

   Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

   Insert

   Pim1 (PIM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 29-313 of isoform 2

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXA9 (murine) FLAG pET

   Plasmid

   #8494

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA9 (Hoxa9 Mouse)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   FLAG epitope tag replaces T7 tag in the pET back…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Use

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHis-hMTMR1

   Plasmid

   #100723

   Purpose

   Expression of human MTMR1 PH-PTP domain

   Depositor

   Byung Il Lee

   Article

   Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

   Insert

   MTMR1 (MTMR1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 95-607

   Promoter

   T7

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM22082_cRed

   Plasmid

   #117225

   Purpose

   pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

   Insert

   Canthaxanthin Operon
   

   Use

   Tags

   34 amino acid N-terminal linker, Hexahistidine Ta…

   Expression

   Bacterial

   Mutation

   removed Type IIS restriction sites within the bac…

   Promoter

   T7 RNA Polymerase

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZM3[I:FruR-L,I:RbsR-L]

   Plasmid

   #60777

   Purpose

   Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   FruR-L
   RbsR-L
   

   Use

   Tags

   Expression

   Mutation

   LacI/GalR repressor chimera. LacI DBD with FruR L…

   Promoter

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3I91

    Plasmid

    #62514

    Purpose

    Bacterial expression plasmid for use in structural determination.

    Depositor

    Cheryl Arrowsmith

    Article

    Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pETNKI-his-3C-LIC-kan

    Plasmid

    #108703

    Purpose

    Expression of an His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETNKI-his-3C-LIC-Amp

    Plasmid

    #108704

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NP-11-63

    Plasmid

    #234358

    Purpose

    Expressing and purifying R.rubrum rubisco using a his-sumo tag

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    N-term SUMO tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p368_DG

    Plasmid

    #234691

    Purpose

    Golden gate domesticated pET28b with 3C-His6

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    3C-His6

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p369_DG

    Plasmid

    #234692

    Purpose

    Golden gate domesticated pET28b with 3C-His10

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    3C-His10

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p344_DG

    Plasmid

    #234674

    Purpose

    Golden gate domesticated pET28b with His6-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p345_DG

    Plasmid

    #234675

    Purpose

    Golden gate domesticated pET28b with His10-3C

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-3C

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p324_DG

    Plasmid

    #234690

    Purpose

    Golden gate domesticated pET28b with TEV-His6

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    TEV-His6

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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