We narrowed to 6,003 results for: 338

1. pRK5myc RhoA L63

   Plasmid

   #15900

   Depositor

   Alan Hall

   Article

   Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Q63L

   Promoter

   Available since

   Nov. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. VPS13D^Halo

   Plasmid

   #174108

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   With an internal Halo tag in aminoacid position 1…

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS426-GFP-Osh2-PH

   Plasmid

   #71373

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh2-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa 277-398

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. IBMc090

   Plasmid

   #161937

   Purpose

   Carries engineered transposon for IBM

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

   Plasmid

   #214998

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

   Plasmid

   #214999

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRIME-CMV-GFP-recipient

   Plasmid

   #11657

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti Vp64-dCas9 T2A mTagBFP2

   Plasmid

   #171052

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   Vp64-dCas9 T2A mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMD18-T-pyrF

   Plasmid

   #174388

   Purpose

   modified pMD18-T containing pyrF and its native promoter, Ampr

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   pyrF
   

   Use

   Template for cloning fragments for chromosomal in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3_PKA-RIIα

    Plasmid

    #168492

    Purpose

    Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    mouse PKA regulatory subunit IIα, untagged
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLJM60-eIF4E

    Plasmid

    #38239

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    eIF4E (Eif4e Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEYFP-N1-HsSmg5_H

    Plasmid

    #146474

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg5 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three non silent mutation A254G (K85R), T980C (L3…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKSB-609 U6-sgRNA1 ATCC GGAA

    Plasmid

    #173671

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDM_noro_A2_lacZA

    Plasmid

    #118819

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKSB-610 U6-sgRNA2 GGAA AGAG

    Plasmid

    #173672

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX2TK-Osh2-PH

    Plasmid

    #71370

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLenti MS2-p65-HSF1 sfGFP

    Plasmid

    #171051

    Purpose

    Expression of Vp64-dCas9 activation system

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    MS2-p65-HSF1_sfGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p.UTA.2.0 Empty

    Plasmid

    #82446

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFP

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTopo pLox hPGK-Puro pA pLox

    Plasmid

    #171048

    Purpose

    Empty donor template for CRISPR/Cas9 targeting of TERT RE's

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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