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mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

Plasmid

#73266

Purpose

Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Plasmid

#159692

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Depositor

Dominic Esposito

Article

Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

M188L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Nterm (23-504) pEF6-V5

Plasmid

#73270

Purpose

Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Mutation

deleted amino acids 505-938

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pB-EFS-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1515)

Plasmid

#242600

Purpose

A PiggyBac expression vector containing the core promoter from human EF1a driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-ActinVhh-sfGFP-P2A-HA-tdiRFP-caax (JDW 1532)

Plasmid

#242606

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin Vhh-sfGFP-P2A-HA-tdiRFP-caax

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnas

Plasmid

#245994

Purpose

Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnas (Gnas Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-hs_UbiC-pro (JDW 1461)

Plasmid

#242547

Purpose

Enhancer/Promoter: Gateway 5' entry vector containing human Ubc promoter (-1225 to -6)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Ubiquitin / Ubc promoter (UBC Human)

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-Myc (JDW 1441)

Plasmid

#242569

Purpose

Gateway middle entry clone containing EGFP fused to oncogenic c-Myc.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-Myc (MYC Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

Plasmid

#170193

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

Plasmid

#170194

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

Plasmid

#170189

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

Plasmid

#229810

Purpose

A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-AmCyan-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

Plasmid

#229814

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

Plasmid

#159697

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

Plasmid

#159679

Purpose

Mammalian protein expression of KRAS4b-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4b (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M88-303: CMV51p> Halotag-Hs.HRAS

Plasmid

#159681

Purpose

Mammalian protein expression of HRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.HRAS (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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