We narrowed to 1,917 results for: FRT
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Plasmid#133613PurposeNS3aH1-VPRDepositorInsertNS3aH1-VPR
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid with onboard mCherry cassette
Plasmid#169741PurposeCMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_HEN1
Plasmid#167708PurposeExpresses HEN1 in mammalian cellsDepositorAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GWF148
Plasmid#133617PurposeGNCR1-BFP-CAAXDepositorInsertGNCR1-BFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF135
Plasmid#133614PurposeANR-ANR-BFP-CAAXDepositorInsertANR-ANR-BFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSU314
Plasmid#169696PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU312
Plasmid#169694PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSHSP:FLP;CMV:tdTomato-SceI
Plasmid#30523DepositorInsertFLP recombinase tdTomato
UseFlp/frt, heat shock inducible, i-scei megabase ve…TagsExpressionMutationR415HPromoterAvailable SinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHW1133
Plasmid#229869PurposeDestination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCEDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_TLX1
Plasmid#167730PurposeExpresses TLX1 in mammalian cellsDepositorAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
OpenLoopControl
Plasmid#59895Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTRDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPESTExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B-AID-EYFP
Plasmid#47329Purposefor plasmid integration using the Flp-In system. AID at C-terminalDepositorAvailable SinceAug. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
PSD-95 FLAG
Plasmid#15463DepositorAvailable SinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA, EGFP Spastin M87 with exon 4
Plasmid#167542PurposeFor doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.DepositorInsertSpastin M87 with exon 4 (SPAST Human)
UseTagsEGFP and HA tagExpressionMammalianMutationPromoterCMVAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid
Plasmid#169732PurposeEqualizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG minimal CMV pCDNA5
Plasmid#212044PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only