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Showing: 1441 - 1460 of 1515 results

  5. pLVX-mCherry-KATNA1

    Plasmid
    #180650
    Purpose
    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    KATNA1 (KATNA1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    siRNA resistant to: GGACAGCACUCCCUUGAAA
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180650-plasmid-map-sequence-id-358623

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  8. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  9. pcDNA3.2-Cx43-ML-HA

    Plasmid
    #49857
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. Cont…
    Promoter
    CMV
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pLAP-BUBR1-3A

    Plasmid
    #114054
    Purpose
    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification
    Depositor
    Geert Kops
    Article
    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:
    Insert
    BUBR1-3A (BUB1B Human)
    Use
    Tags
    YFP-TEV-S- Human LAP
    Expression
    Mammalian
    Mutation
    C2823A and G2826A (siRNA-resistance), BUBR1-S670A…
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    114054-plasmid-map-sequence-id-233658

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  16. pLAP-BUBR1-3D

    Plasmid
    #114055
    Purpose
    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification
    Depositor
    Geert Kops
    Article
    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:
    Insert
    BUBR1-3D (BUB1B Human)
    Use
    Tags
    YFP-TEV-S- Human LAP
    Expression
    Mammalian
    Mutation
    C2823A and G2826A (siRNA-resistance), BUBR1-S670D…
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    114055-plasmid-map-sequence-id-233657

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  17. 5` CC: Puro – loxP – GFP-C

    Plasmid
    #219563
    Purpose
    5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    hPGK-promoter_PuroR_2A_loxP_GFP-C
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219563-plasmid-map-sequence-id-438333

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  18. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Article
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52255-plasmid-map-sequence-id-80847

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Showing: 1441 - 1460 of 1515 results