Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 3,240 results for: Streptococcus

Clear keywords
Showing: 1441 - 1460 of 3240 results

  2. p426_Cas9_gRNA-ARS1622b

    Plasmid
    #87403
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87403-plasmid-map-sequence-id-193446

    Enlarge

  3. p426_Cas9_gRNA-YPRCd15c

    Plasmid
    #87404
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87404-plasmid-map-sequence-id-193447

    Enlarge

  4. p426_Cas9_gRNA-ARS106a

    Plasmid
    #87382
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87382-plasmid-map-sequence-id-193424

    Enlarge

  5. p426_Cas9_gRNA-ARS308a

    Plasmid
    #87384
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87384-plasmid-map-sequence-id-193426

    Enlarge

  6. pADH137

    Plasmid
    #90986
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    90986-plasmid-map-sequence-id-180519

    Enlarge

  9. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    124811-plasmid-map-sequence-id-243700

    Enlarge

  10. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid
    #178091
    Purpose
    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    178091-plasmid-map-sequence-id-350141

    Enlarge

  11. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

    Enlarge

  13. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid
    #173206
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173206-plasmid-map-sequence-id-339514

    Enlarge

  14. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

    Enlarge

  15. p426_Cas9_gRNA-ARS911b

    Plasmid
    #87394
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87394-plasmid-map-sequence-id-193437

    Enlarge

  16. p426_Cas9_gRNA-ARS1309a

    Plasmid
    #87399
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87399-plasmid-map-sequence-id-508300

    Enlarge

  17. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

    Enlarge

  19. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

    Enlarge

  20. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

    Enlarge

Showing: 1441 - 1460 of 3240 results