We narrowed to 16,045 results for: lic

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pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPR - Amplicon, JAK2 sgRNA 1

Plasmid

#70679

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R

Plasmid

#166810

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL1193EE, P1214R

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163064

Purpose

Entry vector for: GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 C-terminal half

Plasmid

#74599

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

amino acids 389-523 of LIC1

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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hSlo2.1 Slick/pCDNA3

Plasmid

#197317

Purpose

Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

Depositor

Christopher Lingle

Insert

Slo2.1 (Kcnt2 Mouse)

Tags

7xHis

Expression

Mammalian

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-Silicatein-pET28

Plasmid

#198012

Purpose

C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

Depositor

Bryan Berger

Article

Vigil et al Materials Advances 2, 2023

Insert

eGFP-silicatein

Tags

hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HLICNot

Plasmid

#140181

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

pEU3-NII-HLICNot

Promoter

T7

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot

Plasmid

#140182

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-FLAGLICNot

Plasmid

#140184

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

FLAG

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-His

Plasmid

#140187

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

6xHis and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR - Amplicon, JAK2 sgRNA 2

Plasmid

#70660

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pRSETC-RdgBbeta-splice variant 1

Plasmid

#58276

Purpose

bacterial expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

Plasmid

#73268

Purpose

[Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163064

Purpose

Entry vector for: GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-human LIC1 C-terminal half

Plasmid

#74599

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

amino acids 389-523 of LIC1

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
hSlo2.1 Slick/pCDNA3

Plasmid

#197317

Purpose

Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

Depositor

Christopher Lingle

Insert

Slo2.1 (Kcnt2 Mouse)

Tags

7xHis

Expression

Mammalian

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-Silicatein-pET28

Plasmid

#198012

Purpose

C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

Depositor

Bryan Berger

Article

Vigil et al Materials Advances 2, 2023

Insert

eGFP-silicatein

Tags

hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge