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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAT024

Plasmid

#171636

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BFP
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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JAB3312

Plasmid

#190406

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3494

Plasmid

#190418

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2602

Plasmid

#190389

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway Cloning

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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ED_p_044b

Plasmid

#246072

Purpose

ECOR31 mCpol D57A bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT3-neo

Plasmid

#250304

Purpose

Encodes gRNA targeting FUT3

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT3 gRNA (FUT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT5-neo

Plasmid

#250305

Purpose

Encodes gRNA targeting FUT5

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT5 gRNA (FUT5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(pre-cut)AB

Plasmid

#248942

Purpose

Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(pre-cut)AB

Expression

Bacterial

Mutation

HamM programmed breaks in the peptide backbone at…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(-cJun)B

Plasmid

#248940

Purpose

Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

flHamMA(-cJun)B

Expression

Bacterial

Mutation

c-Jun insertion, HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(S157A)(deltaA)B

Plasmid

#248939

Purpose

Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(S157A)(deltaA)B

Expression

Bacterial

Mutation

S157A, HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(S157A)(-cJun)B

Plasmid

#248941

Purpose

Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker and the protease mutated (S157A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(S157A)(-cJun)B

Expression

Bacterial

Mutation

S157A, c-Jun insertion, HamA domain deletion

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1583_ECOR31HamA(E138AK140A)B_CoPurification

Plasmid

#223372

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Tags

MBP-His-TEV

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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