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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ACTB_sgRNA

Plasmid

#183884

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ttr

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-eGFP-3HA

Plasmid

#108390

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-3HA

Plasmid

#108395

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Use

Tags

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP-3HA

Plasmid

#108393

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-3HA

Plasmid

#108392

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-eGFP

Plasmid

#108391

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-Jaws-KGC-tdTomato-ER2

Plasmid

#153535

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

Syn

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-MCS(extended)-Puro (empty vector)

Plasmid

#172603

Purpose

Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The multiple cloning site (MCS) has been edited/e…

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-Jaws-KGC-tdTomato-ER2

Plasmid

#153534

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-iSAM

Plasmid

#211495

Purpose

AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

Depositor

Lesley Forrester, Antonella Fidanza

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

UniSAM insert
TET-ON
Neo Resistance
Insulator

Use

CRISPR and Synthetic Biology

Tags

T2A-mCherry Tag

Expression

Mammalian

Mutation

Promoter

EF1a, NA, Splicing acceptor, and TREGV promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Article

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-noSV40-humanEC1.45_min1-2

Plasmid

#173952

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S3-4-5-6

Plasmid

#173956

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 WT

Plasmid

#184627

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)

Use

Tags

Expression

Mammalian

Mutation

no mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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