Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,671 results for: Ado;

Showing: 2191 - 2220 of 4671 results

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pLenti-PGK-RAP2A-N17-neo

Plasmid

#156179

Purpose

Lentiviral vector for expression of RAP2A-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_9

Plasmid

#60295

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCMVblast_SLC38A2_N82A

Plasmid

#156181

Purpose

CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

CMV

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_N82A

Plasmid

#156183

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_29

Plasmid

#60309

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFP36L2 enhancer (ZFP36L2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_10

Plasmid

#60259

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

RFX6 enhancer (RFX6 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_25

Plasmid

#60268

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_31

Plasmid

#60310

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_21

Plasmid

#60296

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CDKN2BAS enhancer (CDKN2B-AS1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_19

Plasmid

#60302

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

THADA enhancer (THADA Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
TDP-43_CTD_12S→E_S305C/A326P

Plasmid

#248868

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with 12S→E, S305C and A326P.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_12S→E_S305C/A326P (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S292E, S369E, S377E, S379E, S387E, S389E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_13S→E_S317C

Plasmid

#248845

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S317C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S317C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_13S→E_S273C

Plasmid

#248859

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S273C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S273C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_12S→E_S305C

Plasmid

#248867

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with 12S→E and S305C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_12S→E_S305C (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S292E, S369E, S377E, S379E, S387E, S389E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-NES-SomaFRCaMPi

Plasmid

#232836

Purpose

AAV transfer plasmid for CAG-mediated Cre-dependent expression of soma-targeted FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomaKGECO1

Plasmid

#232839

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted KGECO1

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaKGECO1 (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-FRCaMPi-P2A-mGreenLantern

Plasmid

#232841

Purpose

AAV transfer plasmid for CAG-mediated co-expression of FRCaMPi and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-NES-KGECO1-P2A-mGreenLantern

Plasmid

#232843

Purpose

AAV transfer plasmid for CAG-mediated co-expression of KGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-KGECO1 (NES Synthetic)
mGreenLantern

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-NES-jRGECO1-P2A-mGreenLantern

Plasmid

#232844

Purpose

AAV transfer plasmid for CAG-mediated co-expression of jRGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-jRGECO1 (NES Synthetic)
mGreenLantern

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-2C-NLS

Plasmid

#239816

Purpose

Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

Rfx-Cas13d

Use

CRISPR

Tags

Nucleoplasmin long NLS and SV40 NLS

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166D

Plasmid

#226720

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166D

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166D

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166L

Plasmid

#226721

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166L

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166L

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-CNDP2-D195A

Plasmid

#226722

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant D195A

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

D195A

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-CNDP2-H445F

Plasmid

#226723

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant H445F

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

H445F

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GSDMD_H270A

Plasmid

#224701

Purpose

Expression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_H270A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

H270A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-GSDMD_Q29A

Plasmid

#224702

Purpose

Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_Q29A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Q29A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-GSDMD_Q29A_H270A

Plasmid

#224703

Purpose

Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_Q29A_H270A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Q29A and H270A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-GSDMD_D275A

Plasmid

#224704

Purpose

Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_D275A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

D275A

Promoter

CVM

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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