We narrowed to 9,222 results for: Pol

1. H1.224.M.luc

   Plasmid

   #193705

   Purpose

   Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2_UBE2:eG-Lt-CsA

   Plasmid

   #136134

   Purpose

   Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpUBE2:eGFP-Lti6b
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN716 - pCAGGS-eGFP-3Xnls-bpA

   Plasmid

   #156435

   Purpose

   For transient expression of nuclear eGFP in mammalian cells

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   eGFP
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L2_UBE2:mT-N7-CsA

   Plasmid

   #136133

   Purpose

   Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpUBE2:mTurq-N7
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET28a-GGG-ELP(120nm)-Cys

   Plasmid

   #91572

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(120nm)-Cys
   

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-Dual_FluA/H7N9

   Plasmid

   #229860

   Purpose

   pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

   Depositor

   Stephen Cusack

   Article

   Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

   Inserts

   PA_H7N9-FluA
   PB1_H7N9-FluA
   PB2_H7N9-FluA
   

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Cys-ELP(120nm)-LPETGG

   Plasmid

   #90472

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(120nm)-LPETGG
   

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Promoter

   TDH3 and TEF1

   Available Since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-miniSOG

   Plasmid

   #193913

   Purpose

   Control plasmid that expresses non-targeted miniSOG in mammalian cells

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MCV-Rep-

    Plasmid

    #32058

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-Rep- genome
    

    Mutation

    Single cytosine to adenine (C/A) mutation at 2,07…

    Promoter

    none from vector. Insert contains native promoter…

    Available Since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWzyE-His

    Plasmid

    #196669

    Purpose

    Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Tags

    TEV cleavable His10

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2pA ins:R198P mVenus

    Plasmid

    #210519

    Purpose

    Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

    Depositor

    Jonathan Rocheleau

    Article

    Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

    Insert

    Apollo-NADP+
    

    Use

    Zebrafish expression, tol2 kit gateway-compatible

    Tags

    mVenus

    Mutation

    R198P

    Promoter

    Zebrafish insulin promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-fsr1

    Plasmid

    #179866

    Purpose

    Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    Fusarubin polyketide synthase
    

    Use

    Synthetic Biology

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-miniSOG-C-Far

    Plasmid

    #193914

    Purpose

    Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H1.224.luc

    Plasmid

    #193704

    Purpose

    Expresses a truncated PolII H1 promoter (H1.244)

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    Truncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-2T-RNAPII-A2-14-HA

    Plasmid

    #160689

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA polymerase II CTD
    

    Expression

    Bacterial

    Mutation

    Contains an S-to-A mutation in position 2 of 14 o…

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p(A)n-PAUSE

    Plasmid

    #118068

    Purpose

    Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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