We narrowed to 6,529 results for: nls

1. pINDUCER20-Flag-NLS-hSPDEF delta3

   Plasmid

   #100876

   Purpose

   Lentiviral expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 243-335aa

   Promoter

   TRE2

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV7.1-Flag-NLS-hSPDEF delta3

   Plasmid

   #100872

   Purpose

   Mammalian expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 244-335aa

   Promoter

   CMV

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV7.1-Flag-NLS-hSPDEF delta1

   Plasmid

   #100870

   Purpose

   Mammalian expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 1-248aa

   Promoter

   CMV

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

   Plasmid

   #68276

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS(4E) mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   RPS2

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPC-Flag-CHES1L-DBD-NLS

   Plasmid

   #51841

   Purpose

   Flag-CHES1L mutant encoding the DNA binding and NLS domains only

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Flag and NLS

   Mutation

   contains CHES1 amino acids 110-205

   Available Since

   July 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. SPL11p::pCGTAG (SPL11p::NLS-GFP)

   Plasmid

   #27411

   Depositor

   David Bartel

   Article

   Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

   Insert

   SPL11 promoter (AT1G27360 Mustard Weed)
   

   Mutation

   SPL11 promoter transcriptionally fused to a nucle…

   Available Since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

   Plasmid

   #115141

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Orf2mor anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-FNLS-P2A-GFP-PGK-Puro

   Plasmid

   #110869

   Purpose

   Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid

    #60367

    Purpose

    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI-D10R-E48R
    

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    D10R+E48R; catalyticaly inactive

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

    Plasmid

    #127659

    Purpose

    Over-express EGFP-NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP-NLS-Flag

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL6puro-JeT-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240106

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added JeT Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240112

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added SV40-Enhancer

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

    Plasmid

    #140534

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-NLS TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

    Plasmid

    #180585

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

    Plasmid

    #203541

    Purpose

    gRNA expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-GFP, 2x U6-RNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140220

    Purpose

    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1
    

    Use

    CRISPR

    Tags

    miniVPR-mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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