We narrowed to 5,839 results for: pCas

1. pCAG-FIP200 (1-640)

   Plasmid

   #203542

   Purpose

   Make FIP200 NTD:ATG13:ULK1 complex

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 AMSHLP 16-218

   Plasmid

   #180609

   Purpose

   Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   AMSHLP residues 16-218
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 16-218

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 KATNAL2 residues 1-98

   Plasmid

   #180619

   Purpose

   Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNAL2 (KATNAL2 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-98

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Myc-KATNB1

   Plasmid

   #160054

   Purpose

   Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNB1 (KATNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-C-TEV-HAT

   Plasmid

   #202597

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Tags

   TEV cleavable site (ENLYFQG) and poly-histidine a…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-N-TEV-HAT

   Plasmid

   #202598

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Tags

   TEV cleavable site (ENLYFQG) and poly-histidine a…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

   Plasmid

   #197421

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

   Plasmid

   #197422

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

    Plasmid

    #197424

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas-Cas12m-ΔZF

    Plasmid

    #192276

    Purpose

    Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    Cas12m ΔZF
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    H459A, C552A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas-dCas12m-ΔZF

    Plasmid

    #192277

    Purpose

    pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m ΔZF
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A, H549A, C552A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-DIO-basic

    Plasmid

    #196882

    Purpose

    Used for preparation of double-floxed inverse ORF vectors

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Type

    Empty backbone

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-1700029I15Rik-3×FLAG

    Plasmid

    #199689

    Purpose

    Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

    Insert

    1700029I15Rik (1700029I15Rik Mouse)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG.Myc-SARS2_3CL(C145A)

    Plasmid

    #200121

    Purpose

    Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    CAG

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG1.1-Tsks/1D4

    Plasmid

    #199623

    Purpose

    Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Tsks (variant X6) (Tsks Mouse)
    

    Tags

    1D4

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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