161,377 results

1. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRyl_D17

   Plasmid

   #84611

   Purpose

   Introduces DSB at D17 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DYRK1A-2A-GFP

   Plasmid

   #118270

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GBX

   Plasmid

   #64123

   Purpose

   a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

   Depositor

   Linzhao Cheng

   Article

   Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

   Insert

   GFP-2A-BCL2L1 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGD283

   Plasmid

   #129733

   Purpose

   Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.

   Depositor

   Rüdiger Simon

   Article

   Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD44S pWZL-Blast

   Plasmid

   #19126

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   CD44 standard (CD44 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 p53 WT

   Plasmid

   #69003

   Purpose

   mammalian expression of wild type human p53

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. inert_EJC

   Plasmid

   #216224

   Purpose

   GFP P2A RFP reporter with inert EJC immediately following stop codon

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_inert_EJC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIS1-Eef25UTR-renilla

    Plasmid

    #38235

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2 5'UTR / Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Eef2 5' UTR fused to renilla ORF

    Promoter

    Endogenous Eef2

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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