We narrowed to 11,192 results for: NSI

1. sgRNA2 _1xCTS-ECFP-pA

   Plasmid

   #200238

   Purpose

   Mammalian transfections; 1xCTS-ECFP reporter construct 2

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA2_1xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. vYM143_phiC31-nTRE-pEF1s(low)(CG793)-H2b-Citrine

   Plasmid

   #197686

   Purpose

   "pEF1s(low)" reporter plasmid, with CpG793 mutated to CG

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   pEF1s-H2B-Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine

   Plasmid

   #197678

   Purpose

   "pEF1s(high)" reporter plasmid

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   pEF1s-H2B-Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. vYM011_phiC31-nTRE-pEF1s(low)-H2b-Citrine

   Plasmid

   #197679

   Purpose

   "pEF1s(low)" reporter plasmid

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   pEF1s-H2B-Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine

   Plasmid

   #197684

   Purpose

   "pEF1s(orig)" reporter plasmid, with CpG793 mutated to CC

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   pEF1s-H2B-Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. myr-palm-Gamillus

   Plasmid

   #197920

   Purpose

   Monomer expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   glycosylphosphatidylinositol-Gamillus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET51b_SNAP-ecDHFRmt

   Plasmid

   #187109

   Purpose

   Expression of SNAP-ecDHFRmt fusion in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   SNAP-ecDHFRmt
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   ecDHFR-R44L-H45Q

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYCTK010

   Plasmid

   #176714

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   mfalpha1Ka
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Codon-optimized for expression in S. cerevisiae

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BiDi-[mp-mEGFP]mp-mCherry2

   Plasmid

   #182870

   Purpose

   Bi-directional expression vector of monomers with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

   Insert

   myr-palm-mEGFP, myr-palm-mCherry2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A0A0W0YYP4

    Plasmid

    #163267

    Purpose

    Inducible expression of UniProt identifier A0A0W0YYP4

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    A0A0W0YYP4
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. U2ENB5

    Plasmid

    #163292

    Purpose

    Inducible expression of UniProt identifier U2ENB5

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    U2ENB5
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. A0A0R3K2G2

    Plasmid

    #163263

    Purpose

    Inducible expression of UniProt identifier A0A0R3K2G2

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    A0A0R3K2G2
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M7MTF7

    Plasmid

    #163282

    Purpose

    Inducible expression of UniProt identifier M7MTF7

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    M7MTF7
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. W6I5R7

    Plasmid

    #163295

    Purpose

    Inducible expression of UniProt identifier W6I5R7

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    W6I5R7
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYCTK013

    Plasmid

    #176717

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea fermentati (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Lf
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYCTK021

    Plasmid

    #176725

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kazachstania africana (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Ka
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYCTK022

    Plasmid

    #176726

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kluyveromyces lactis (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Kl
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYCTK023

    Plasmid

    #176727

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kazachstania naganishii (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Kn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYCTK024

    Plasmid

    #176728

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea fermentati (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Lf
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYCTK026

    Plasmid

    #176730

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea thermotolerans (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Lt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYCTK029

    Plasmid

    #176733

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Vanderwaltozyma polyspora (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Vp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYCTK032

    Plasmid

    #176736

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Kluyveromyces lactis (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Kl
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYCTK033

    Plasmid

    #176737

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Kazachstania naganishii (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Kn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYCTK017

    Plasmid

    #176721

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Tetrapisispora phaffii (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Tp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYCTK012

    Plasmid

    #176716

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania naganishii (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Kn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYCTK018

    Plasmid

    #176722

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Vanderwaltozyma polyspora (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Vp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYCTK015

    Plasmid

    #176719

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea thermotolerans (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Lt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYCTK014

    Plasmid

    #176718

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea mirantina (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Lm
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYCTK011

    Plasmid

    #176715

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kluyveromyces lactis (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Kl
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TetO::EGFP-C-Meikin

    Plasmid

    #174706

    Purpose

    Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    155-373

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRSETA_crG18

    Plasmid

    #172930

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorG18
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PAJ408

    Plasmid

    #104150

    Purpose

    dCas9 plasmid expressing full length mKate2 mRNA without RBS

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. lentiCRISPR v2-sgSmg7

    Plasmid

    #161809

    Purpose

    guide RNA for Smg7

    Depositor

    Yi Zhang

    Article

    Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

    Insert

    sgRNA targeting mouse Smg7 (Smg7 Mouse, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCL27-decoder

    Plasmid

    #159449

    Purpose

    Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1tet-citrine-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1tet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ROPY-4A

    Plasmid

    #128005

    Purpose

    Unphosphorylatable mutant of Stathmin/OP18 FRET-sensor

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

    Insert

    ROPY-4A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S16A, S25A, S38A, S63A in OP18

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ROPY-4E

    Plasmid

    #128006

    Purpose

    Phosphomimic mutant of Stathmin/OP18 FRET-sensor

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

    Insert

    ROPY-4E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S16E, S25E, S38E, S63E in OP18

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ROPY-sYFP2(Y67G)

    Plasmid

    #128008

    Purpose

    Dark sYFP2 mutant of ROPY

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

    Insert

    ROPY-sYFP2(Y67G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y67G in sYFP2

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. sYFP2-OP18-mScI

    Plasmid

    #128003

    Purpose

    Redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

    Insert

    sYFP2-OP18-mScI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RsCCR2_EYFP_pcDNA3.1

    Plasmid

    #159125

    Purpose

    This plasmid encodes RsCCR2 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

    Insert

    RsCCR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. RsCCR1_EYFP_pcDNA3.1

    Plasmid

    #159124

    Purpose

    This plasmid encodes RsCCR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

    Insert

    RsCCR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge