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Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only
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GFP-NPM NLS1D
Plasmid#13284DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa134-aa142PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1/2D
Plasmid#13287DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa134-aa142, aa150-aa155PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NESM-NLS1D
Plasmid#13288DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationL100A, L102A, deletion of aa134-aa142PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTORKm43GWC
Plasmid#112898PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-th-sgRNA
Plasmid#65565Purposein vitro trancription of sgRNA targeting the zebrafish th locusDepositorInsertth sgRNA (th Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NESD
Plasmid#13283DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa94-aa102PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTORKm43GWY
Plasmid#112901PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#164046PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA geneDepositorInsertmTagBFG-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUltra-5HTP
Plasmid#178042PurposeExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codonDepositorInsertsModified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation
UseTagsExpressionBacterialMutationT107C, P254T, C255APromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS2D
Plasmid#13285DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa150-aa155PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
LDB204
Plasmid#185426PurposeBacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusionDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 FXFG-repeats domain expressedPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239A (dFnCas12a)-TV
Plasmid#136379PurposeCRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation systemDepositorInsertdFnCas12a-TV
UseCRISPRTagsExpressionPlantMutationD917APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-CDS1-3HA
Plasmid#198596PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorInsertCDS1 (CDS1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorInsertDGK1 (DGK1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AT1R DRY-AAY
Plasmid#45759DepositorInsertAT1R DRY-AAY (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationchanged amino acids 125-126 from DR to AAPromoterCMVAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertABF2 (ABF2 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8
Plasmid#136376PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-Halo donor
Plasmid#197866PurposeDonor plasmid used for building MED6-Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsHalotagExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZyxin-mMaple2
Plasmid#101150PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorInserthuman codon optimized mMaple2 fused to zyxin
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationD570A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.4
Plasmid#136375PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291
Plasmid#129671PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationBthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …TagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ293 (BhCas12b_v4)
Plasmid#136383PurposeGateway entry clone for BhCas12b_v4DepositorInsertBhCas12b_v4
UseCRISPRTagsExpressionPlantMutationK846R/S893R/E837GPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT6-G60A
Plasmid#102326PurposeHuman SIRT6 G60A for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseLentiviralTagsNoneExpressionMammalianMutationchanged glycine 60 to alaninePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBOB-HA-Gnaq
Plasmid#236750PurposeExpress HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorInsertGnaq (Gnaq Mouse)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BirA-HA-Tulp3
Plasmid#236749PurposeExpress BirA and HA tagged TULP3 transiently via transfection in mammalian cells.DepositorInsertTulp3 (Tulp3 Mouse)
UseTagsBirA and HAExpressionMammalianMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGC-Lb12-P1Bb
Plasmid#231387PurposeFor crRNA cloning with LbCas12a vectors for genome editing in ArabidopsisDepositorInsertU6-29
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-mCherry-mDYNLL1
Plasmid#210336PurposeOverexpression of mDYNLL1DepositorInsertmDYNLL1 (Dynll1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-mEmerald donor
Plasmid#197865PurposeDonor plasmid used for building MED6-mEmerald knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-Oct4
Plasmid#197909Purposesingle-particle tracking for OCT4DepositorInsertpou5f1 (Pou5f1 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterPAH1Available sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-CDS1-3HA
Plasmid#184760PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorInsertCDS1 (CDS1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterCDS1Available sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-DGK1-3HA
Plasmid#184759PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorInsertDGK1 (DGK1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterDGK1Available sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
OM14-2EGFP-UG74-ter
Plasmid#184758PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertOM14 (OM14 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2EGFP-ter-NAT
Plasmid#184764PurposeMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE1-2EGFP-ter-kanMX4
Plasmid#184766PurposeIntegration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.DepositorInsertIRE1 (IRE1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIT1-2EGFP-ter-URA3
Plasmid#184767PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertCIT1 (CIT1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertEMC1 (EMC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACH1-2EGFP-ter-URA3
Plasmid#184769PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertACH1 (ACH1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFG3-2EGFP-ter-URA3
Plasmid#184770PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertAFG3 (AFG3 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYS1-2EGFP-ter-URA3
Plasmid#184771PurposeMarker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertSYS1 (SYS1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only