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Showing: 3701 - 3750 of 52550 results

  1. pEGFP-N1-GRASP55

    Plasmid
    #137708
    Purpose
    Eukaryotic expression of rat GRASP55 full-length
    Depositor
    Yanzhuang Wang
    Insert
    GRASP55
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    137708-plasmid-map-sequence-id-273547

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  3. pvPE-V4-Puro

    Plasmid
    #240290
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4-Puro
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240290-plasmid-map-sequence-id-488804

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  4. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  10. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

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  11. FLINC-AKAR1(TA)-CAAX

    Plasmid
    #87706
    Purpose
    Negative-control mutant of FLINC-AKAR1-CAAX.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1(TA)-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87706-plasmid-map-sequence-id-171088

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  12. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  18. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  19. pvPE-V3-Puro

    Plasmid
    #240289
    Purpose
    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V3-Puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240289-plasmid-map-sequence-id-488806

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  20. pBOB-HA-Gnas

    Plasmid
    #245994
    Purpose
    Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gnas (Gnas Mouse)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245994-plasmid-map-sequence-id-488657

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  21. Cd99l2 long

    Plasmid
    #74469
    Purpose
    Expresses Cd99l2 transcript variant 1-IRES-EGFP under CAG promoter in mammalian cells
    Depositor
    Young Ho Suh
    Article
    Kang et al Cell Rep. 2025 Jan 28;44(1):115155. doi:
    Insert
    CD99 antigen-like 2, transcript variant 1 (Cd99l2 Mouse)
    Expression
    Mammalian
    Promoter
    chicken beta actin promoter
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only

  22. Cd99l2 short

    Plasmid
    #74470
    Purpose
    Expresses Cd99l2 transcript variant 2-IRES-EGFP under CAG promoter in mammalian cells
    Depositor
    Young Ho Suh
    Article
    Kang et al Cell Rep. 2025 Jan 28;44(1):115155. doi:
    Insert
    CD99 antigen-like 2, transcript variant 2 (Cd99l2 Mouse)
    Expression
    Mammalian
    Promoter
    chicken beta actin promoter
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only

  24. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Depositor
    Abhishek Chatterjee
    Article
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217368-plasmid-map-sequence-id-430295

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  30. KBB528

    Plasmid
    #185104
    Purpose
    C-terminal truncation of Pom152 fused to GFP
    Depositor
    Ken Belanger
    Insert
    POM152
    Tags
    GFP
    Expression
    Yeast
    Mutation
    POM152(1-???)-HA-GFP
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185104-plasmid-map-sequence-id-368165

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  31. LDB361

    Plasmid
    #185435
    Purpose
    Bacterial expression of full length NUP2 as fusion with GST
    Depositor
    Ken Belanger, Laura Davis
    Insert
    NUP2
    Tags
    GST
    Expression
    Bacterial
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185435-plasmid-map-sequence-id-367560

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  32. KBB609

    Plasmid
    #185110
    Purpose
    Pom152 N-terminus in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 N-term-6His
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185110-plasmid-map-sequence-id-368108

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  33. pBOB-Mrap2-P2A-Mc4r-HA

    Plasmid
    #245991
    Purpose
    Stimultaneously express HA C-terminal tagged mouse MC4R and untagged MRAP2 protein using a P2A linker, transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Mrap2 and Mc4r (Mrap2, Mc4r Mouse)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245991-plasmid-map-sequence-id-488668

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  34. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

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  35. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  36. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  37. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  40. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  45. pBOB-Gpr45-161-3xFlag

    Plasmid
    #245993
    Purpose
    Express 3xFlag C-terminal tagged mouse GPR45-N and GPR161-C fusion protein transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.
    Insert
    Gpr45-GPR161161 fusion (Gpr161, Gpr45 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    aa 1 to 327 of Gpr45; aa 357 to 558 of Gpr161
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245993-plasmid-map-sequence-id-488725

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  46. 7AA-aSyn-pRK172

    Plasmid
    #236197
    Purpose
    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.
    Depositor
    Sjors Scheres, Yang Yang
    Article
    Yang et al Acta Neuropathol. 2023 May;145(5):561-57
    Insert
    SNCA (SNCA Human)
    Expression
    Bacterial
    Mutation
    insertion os MAAAEKT at residue 22
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236197-plasmid-map-sequence-id-469796

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  48. KBB610

    Plasmid
    #185111
    Purpose
    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 Repeats-6His
    Available Since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185111-plasmid-map-sequence-id-368105

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  49. KBB611

    Plasmid
    #185112
    Purpose
    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 repeats 4&5-6His
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185112-plasmid-map-sequence-id-368078

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  50. LDB223

    Plasmid
    #185428
    Purpose
    Bacterial expression Nup2 N-terminal domain as GST fusion
    Depositor
    Ken Belanger, Laura Davis
    Insert
    NUP2
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup2 N-terminal domain
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185428-plasmid-map-sequence-id-367563

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Showing: 3701 - 3750 of 52550 results